Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604195912_6iyq CAGGACTTTATTTCTTAGCCTGGCTTCTCCTG(+ACTCAC)GTACCGATACCAACGATTTTAAATATGCTTT NGG 10 8 17 8 24 0 100 5 10
    240604195911_5cdh CACAGCCATGCCCAGCTTTTTGTGAGTGCTGG(+ACTCAC)GATCCAAACTCAGGTACTTCTTACCCACTGA NGG 10 8 17 8 24 0 100 5 10
    240604195909_a72m ATGTCTCAGTGGGTAAGAAGTACCTGAGTTTG(+ACTCAC)GATCCCAGCACTCACAAAAAGCTGGGCATGG NGG 10 8 17 8 24 0 100 5 10
    240604195908_limp ACTGTCTTTACACACACTAGAAGAGGGCATTG(+ACTCAC)GATCCCATTACATGTGGTTGCTGGGAATTGA NGG 10 8 17 8 24 0 100 5 10
    240604195906_gp9u TGAGTTCAATTCCCAGCAACCACATGTAATGG(+ACTCAC)GATCCAATGCCCTCTTCTAGTGTGTGTAAAG NGG 10 8 17 8 24 0 100 5 10
    240604195904_phwg TGACCCGTGCTTTGAGAACTCGGGAAAACCAG(+ACTCAC)GATCCATGTCCACTTCACTTCGACTTTATCT NGG 10 8 17 8 24 0 100 5 10
    240604195903_u1sq TGAGGAGATAAAGTCGAAGTGAAGTGGACATG(+ACTCAC)GATCCTGGTTTTCCCGAGTTCTCAAAGCACG NGG 10 8 17 8 24 0 100 5 10
    240604195900_3ovm CATGTAACGGAAGGGTGGGCGCAGATCCTTAG(+ACTCAC)GTACCAGGGTGCTGTGCAATGGAAGATGCAG NGG 10 8 17 8 24 0 100 5 10
    240604195857_hnfa CATCTCTGCATCTTCCATTGCACAGCACCCTG(+ACTCAC)GTACCTAAGGATCTGCGCCCACCCTTCCGTT NGG 10 8 17 8 24 0 100 5 10
    240604195855_324c ACACACACACACACAAATACTCAAGAGGTGGG(+ACTCAC)GATCCACAAATATGAAGTAAACCAGAGCAGT NGG 10 8 17 8 24 0 100 5 10