Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604200020_mmdy ATGGCATTTTGAAGTATGCATTTAACAAGTGG(+ACTCAC)GTACCATGATCTCAGAGGGTGAGTGAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240604200018_uar3 ATTTAAGACATTACCTCCTAGCATTCCAAGGG(+ACTCAC)GATCCCAGATTTGAAAGGGCAAGCCTGGCAT NGG 10 8 17 8 24 0 100 5 10
    240604200016_vifq CATTTATGCCAGGCTTGCCCTTTCAAATCTGG(+ACTCAC)GATCCCCTTGGAATGCTAGGAGGTAATGTCT NGG 10 8 17 8 24 0 100 5 10
    240604200015_562i TTCATTTTAGATTACTAATAAAGGGAGAAAAG(+ACTCAC)GTACCTTCAAAGCTTATTCACATTTGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240604200013_77b5 AAAGATTTTGCAAATGTGAATAAGCTTTGAAG(+ACTCAC)GTACCTTTTCTCCCTTTATTAGTAATCTAAA NGG 10 8 17 8 24 0 100 5 10
    240604200012_6dot TCATTTCCCAGAGGGCGTCAGATGGTGGTATG(+ACTCAC)GATCCTGCCTCCTGAACTCAATCCTTTCAGT NGG 10 8 17 8 24 0 100 5 10
    240604200010_i47i CCTGCACTGAAAGGATTGAGTTCAGGAGGCAG(+ACTCAC)GATCCATACCACCATCTGACGCCCTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240604200008_czzm AGTCATTGTTAGGTGTTCGTGTTGGGAATGGG(+ACTCAC)GATCCTTCAAGGAACAGAGTAAAGCACCTTC NGG 10 8 17 8 24 0 100 5 10
    240604200007_y5vo AGGGTGAAGGTGCTTTACTCTGTTCCTTGAAG(+ACTCAC)GATCCCCATTCCCAACACGAACACCTAACAA NGG 10 8 17 8 24 0 100 5 10
    240604200006_52jg TACACCGGGTCTCTTTAAAATTGGCATGCTCG(+ACTCAC)GTACCAAATAGGTGAGCTCATAATTAGAAAT NGG 10 8 17 8 24 0 100 5 10