Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192627_i1pn CCAAGGAGATGTCTCCAGTGGCCCCATCACTG(+ACTCAC)GTACCCTGAGCCCTTCATCACATTCGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240604192626_v4p0 GTCACAGGGCTGGCATCTGGAACACAGCACAG(+ACTCAC)GTACCAAGACAAGGGTAAAATCTTGTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240604192625_ev7p CTGCTGCTTTACAAGATTTTACCCTTGTCTTG(+ACTCAC)GTACCTGTGCTGTGTTCCAGATGCCAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240604192623_92xd TTTTGAGACACAGGTTTTCTATGGCCTTCCTG(+ACTCAC)GATCCTCATTGTCTCTCTCCCCAACTCACAG NGG 10 8 17 8 24 0 100 5 10
    240604192622_sk2x CTGTTCTGTGAGTTGGGGAGAGAGACAATGAG(+ACTCAC)GATCCAGGAAGGCCATAGAAAACCTGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240604192619_qufe AACGGCCATTGGGAAATTTAGGGAAACCCATG(+ACTCAC)GATCCTAGCTGACAGGAGGAGTAGGGTCATT NGG 10 8 17 8 24 0 100 5 10
    240604192618_rsez GCAGCAATGACCCTACTCCTCCTGTCAGCTAG(+ACTCAC)GATCCATGGGTTTCCCTAAATTTCCCAATGG NGG 10 8 17 8 24 0 100 5 10
    240604192616_asoo ATTGTGCCTTCTGGACCAGGAGAGTAGCTTCG(+ACTCAC)GATCCCCTTGGCTGGTCCAACCTCTACTCCA NGG 10 8 17 8 24 0 100 5 10
    240604192614_w3pn CAGCCTGGAGTAGAGGTTGGACCAGCCAAGGG(+ACTCAC)GATCCGAAGCTACTCTCCTGGTCCAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240604192612_0ouo AAGGGCGGGGCTGGTTTGGCTTTTGTTGTTGG(+ACTCAC)GATCCCCGTGAGTGACAGGTTTTGCCGTGCA NGG 10 8 17 8 24 0 100 5 10