Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192536_8acu TGCACAGTGCCTGGTACCTGAAGAGGGATTTG(+ACTCAC)GATCCTGGGACAGGAATAACAAGACAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240604192533_deco TATATAATATCTTCTTTTTTTTTAAAGCCCCG(+ACTCAC)GATCCAAGGCGTCCAAAGTTATTAAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240604192531_43lx TGAACTTTATTTTAATAACTTTGGACGCCTTG(+ACTCAC)GATCCGGGGCTTTAAAAAAAAAGAAGATATT NGG 10 8 17 8 24 0 100 5 10
    240604192530_i9kz GTAAGATACATAAATATAGAATTAATATAAAG(+ACTCAC)GTACCACTATGTTTTACTTATAATAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240604192528_bzgm AAATGTGTTTTTATTATAAGTAAAACATAGTG(+ACTCAC)GTACCTTTATATTAATTCTATATTTATGTAT NGG 10 8 17 8 24 0 100 5 10
    240604192526_3qyw CAACTGAGAGAAGTGGGAAGAGCATCTGGTGG(+ACTCAC)GTACCAATTGGCAGAGAAGGGGAGAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240604192525_f8au ATGACCAGTCTTCTCCCCTTCTCTGCCAATTG(+ACTCAC)GTACCCACCAGATGCTCTTCCCACTTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240604192524_oe40 TCTCTTCCACTCAGAAAGTGGCATGGCTTCAG(+ACTCAC)GTACCACAGGGCACATATGTCAATCAACCCT NGG 10 8 17 8 24 0 100 5 10
    240604192522_ryzn AGACCAGGGTTGATTGACATATGTGCCCTGTG(+ACTCAC)GTACCTGAAGCCATGCCACTTTCTGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604192521_5s1e CCCATGAGAGTGTCGGGGTCAGGGGCTGAAAG(+ACTCAC)GATCCAACATTTCCAAGTTAGAAGTTAAGCT NGG 10 8 17 8 24 0 100 5 10