Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604192552_wf2k CCAGAAAACAGCACAGTTCTGTTACTCTTGTG(+ACTCAC)GTACCTGGCACCATTTTTTGTTTCTGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604192551_uts8 AGGCCACTTCTCCAAGACAGTTTCTGACACTG(+ACTCAC)GTACCTTCCCCTAATCATGTATCTCCCAAAA NGG 10 8 17 8 24 0 100 5 10
    240604192549_xhjb TCATCTTTTGGGAGATACATGATTAGGGGAAG(+ACTCAC)GTACCAGTGTCAGAAACTGTCTTGGAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240604192547_n7v0 GCTTATCCAGGGCTCACTTTCGTCCTTTGTAG(+ACTCAC)GTACCCGCATTCCTACGCATTCATATACATA NGG 10 8 17 8 24 0 100 5 10
    240604192545_tjvs GTTTGTATGTATATGAATGCGTAGGAATGCGG(+ACTCAC)GTACCTACAAAGGACGAAAGTGAGCCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240604192544_78v4 CAGATTTTAGGTAAGTTTATTTGACTAAATTG(+ACTCAC)GATCCTTTTATATTAACAATTCAGCATCTTT NGG 10 8 17 8 24 0 100 5 10
    240604192542_nd1k TTATAAAAGATGCTGAATTGTTAATATAAAAG(+ACTCAC)GATCCAATTTAGTCAAATAAACTTACCTAAA NGG 10 8 17 8 24 0 100 5 10
    240604192541_9xd4 ATTCCTGTCCCAGGATCCAAATCCCTCTTCAG(+ACTCAC)GTACCAGGCACTGTGCAAAGACACATTCAGG NGG 10 8 17 8 24 0 100 5 10
    240604192540_tju8 TTTTGCCTGAATGTGTCTTTGCACAGTGCCTG(+ACTCAC)GTACCTGAAGAGGGATTTGGATCCTGGGACA NGG 10 8 17 8 24 0 100 5 10
    240604192537_sq4k AGCTCACAACTGTCTTGTTATTCCTGTCCCAG(+ACTCAC)GATCCAAATCCCTCTTCAGGTACCAGGCACT NGG 10 8 17 8 24 0 100 5 10