Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154505_81po TCTGGGCCCATTACCTGCTGTGCATAGGACTG(+AGGACA)GTACCAAGGACTATTGAGTGTAGACAAACGA NGG 10 8 17 8 24 0 100 5 10
    240613154503_q1ax GTTGATCGTTTGTCTACACTCAATAGTCCTTG(+AGGACA)GTACCAGTCCTATGCACAGCAGGTAATGGGC NGG 10 8 17 8 24 0 100 5 10
    240613154501_ql3h TCTGGGTTCAAAACATAAACAAAATATACCCG(+AGGACA)GTACCAAAATAAAATAGTACAAACCAAATAC NGG 10 8 17 8 24 0 100 5 10
    240613154500_viq8 AGCTAGTATTTGGTTTGTACTATTTTATTTTG(+AGGACA)GTACCGGGTATATTTTGTTTATGTTTTGAAC NGG 10 8 17 8 24 0 100 5 10
    240613154458_8is1 TTTAATTTCTAAGAATGTGCATGTGTCTGTGG(+AGGACA)GTACCACTGCCCAATACAGCCAGAAAATGGT NGG 10 8 17 8 24 0 100 5 10
    240613154457_7vf1 CTCATACCATTTTCTGGCTGTATTGGGCAGTG(+AGGACA)GTACCCACAGACACATGCACATTCTTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613154456_eri9 GTACCCTTCCTTTACCCCCAGTGGCTGCTAAG(+AGGACA)GTACCTTTGAAACACAAATCAGATATTTATA NGG 10 8 17 8 24 0 100 5 10
    240613154454_mk49 TGTAATATAAATATCTGATTTGTGTTTCAAAG(+AGGACA)GTACCTTAGCAGCCACTGGGGGTAAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613154453_2rg7 GGTTCTCTCTCACGGATTACCGCTCCTGATAG(+AGGACA)GTACCTGACAGGCTCATTCTATATCCCAATT NGG 10 8 17 8 24 0 100 5 10
    240613154452_ph2w CTCAAAATTGGGATATAGAATGAGCCTGTCAG(+AGGACA)GTACCTATCAGGAGCGGTAATCCGTGAGAGA NGG 10 8 17 8 24 0 100 5 10