Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154519_oouz AATGAGCTACCCAACTTCTACCACATTCCTGG(+AGGACA)GTACCCAACTGTGTTGTTACTCTGAACCCAC NGG 10 8 17 8 24 0 100 5 10
    240613154518_6txt AAACTGTGGGTTCAGAGTAACAACACAGTTGG(+AGGACA)GTACCCAGGAATGTGGTAGAAGTTGGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240613154516_rso0 GAAGAAGCCCAAGAAAAAAGCAGGGTTATCTG(+AGGACA)GTACCTATCCTGGGCAGAATCACAGGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240613154515_jcim ATCAGTACAACCTGTGATTCTGCCCAGGATAG(+AGGACA)GTACCAGATAACCCTGCTTTTTTCTTGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613154514_f7db CACACGTTAAGATGCCTTTCTCGCCTCTGAAG(+AGGACA)GATCCTGAGTCACTGCCCTCATTTACTATGA NGG 10 8 17 8 24 0 100 5 10
    240613154513_416e GGGATTCATAGTAAATGAGGGCAGTGACTCAG(+AGGACA)GATCCTTCAGAGGCGAGAAAGGCATCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240613154511_vpz8 TCAGTCTCAGCACTCAGAGACAGAGCTAGGTG(+AGGACA)GATCCCTGAGTTCGAGGCCAGCCTGGTCTAC NGG 10 8 17 8 24 0 100 5 10
    240613154510_8ct2 TCTCTGTAGACCAGGCTGGCCTCGAACTCAGG(+AGGACA)GATCCACCTAGCTCTGTCTCTGAGTGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240613154508_6uoi GTATTGTTTGTCAGCTCAGAAAGAATCAGGTG(+AGGACA)GTACCCAAGTGCGGGGTCTTCCTAACGTTGA NGG 10 8 17 8 24 0 100 5 10
    240613154506_na13 ATTTTTCAACGTTAGGAAGACCCCGCACTTGG(+AGGACA)GTACCACCTGATTCTTTCTGAGCTGACAAAC NGG 10 8 17 8 24 0 100 5 10