Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154825_ejbv GACATGCCACCCACCGCACTTGGCTTTATCAG(+AGGACA)GATCCATTTGTTGGTAGAAGCAAAAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613154824_m0vs CCAAGAGCTGCTTTTGCTTCTACCAACAAATG(+AGGACA)GATCCTGATAAAGCCAAGTGCGGTGGGTGGC NGG 10 8 17 8 24 0 100 5 10
    240613154823_ml25 CCTTTTCTGTGTGCTGTACATCCCAGTCAGTG(+AGGACA)GTACCTAGCCCAGTTTCTCTCTGGCTGGCAC NGG 10 8 17 8 24 0 100 5 10
    240613154821_r3ob TGATGGTGCCAGCCAGAGAGAAACTGGGCTAG(+AGGACA)GTACCACTGACTGGGATGTACAGCACACAGA NGG 10 8 17 8 24 0 100 5 10
    240613154820_85qq AGGTGCCCATGCTCAGTGGTCAGCTCTGGGTG(+AGGACA)GTACCACGAGGCTCACTCGCGCAATAATGCT NGG 10 8 17 8 24 0 100 5 10
    240613154819_ha01 ATGGCAGCATTATTGCGCGAGTGAGCCTCGTG(+AGGACA)GTACCACCCAGAGCTGACCACTGAGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240613154817_3mh6 TGTCTTAAAGAGTCAATAGGAGGGGCCCAATG(+AGGACA)GATCCCAGGGTGGAGGCCTAAGTTACTCTGG NGG 10 8 17 8 24 0 100 5 10
    240613154814_067l GGCCTCCAGAGTAACTTAGGCCTCCACCCTGG(+AGGACA)GATCCATTGGGCCCCTCCTATTGACTCTTTA NGG 10 8 17 8 24 0 100 5 10
    240613154812_8f7n GAGGGGAAGACGCAGGGCCTCTCAGGATGTGG(+AGGACA)GATCCGGCAGACATCACTGGGGCTGGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240613154810_yn7y TTAACAGGACCCAGCCCCAGTGATGTCTGCCG(+AGGACA)GATCCCACATCCTGAGAGGCCCTGCGTCTTC NGG 10 8 17 8 24 0 100 5 10