Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154728_4vax CTGAAGATGATATTTTGATTTGTATTTTGGGG(+AGGACA)GTACCTGTGTTGAGTCGATAAAACATTTTCA NGG 10 8 17 8 24 0 100 5 10
    240613154727_tuaf GGAGATGAAAATGTTTTATCGACTCAACACAG(+AGGACA)GTACCCCCAAAATACAAATCAAAATATCATC NGG 10 8 17 8 24 0 100 5 10
    240613154725_heuu AACCTCTCCCTAGCAGGCTTCCCTGGGACGGG(+AGGACA)GTACCTTTCCAGTTGGTGTTCCCTGGCTTGT NGG 10 8 17 8 24 0 100 5 10
    240613154724_dugx CACACACAAGCCAGGGAACACCAACTGGAAAG(+AGGACA)GTACCCCGTCCCAGGGAAGCCTGCTAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240613154722_hki1 CGTGTGTTCCTGCCAGCATAGGACCAGGATGG(+AGGACA)GTACCTTCTCCAAGCTCCGCAGAGGAGGACC NGG 10 8 17 8 24 0 100 5 10
    240613154721_8nld GCTGGGGTCCTCCTCTGCGGAGCTTGGAGAAG(+AGGACA)GTACCCATCCTGGTCCTATGCTGGCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240613154718_c4bd CCCAAATCAAAAGAAAATTCACATTTGCCCTG(+AGGACA)GATCCCAGACATAGACATATACAGTCATACA NGG 10 8 17 8 24 0 100 5 10
    240613154716_ql56 CCTCTTGTATGACTGTATATGTCTATGTCTGG(+AGGACA)GATCCAGGGCAAATGTGAATTTTCTTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240613154715_im19 GCAGAAAGTCCAGTGGCGACCCCATATGGCAG(+AGGACA)GATCCCGATTTGTAGGTCACCAGCATATGGT NGG 10 8 17 8 24 0 100 5 10
    240613154713_jjl8 TGATGACCATATGCTGGTGACCTACAAATCGG(+AGGACA)GATCCTGCCATATGGGGTCGCCACTGGACTT NGG 10 8 17 8 24 0 100 5 10