Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154746_ru6q AGCCCTGTTAGGGAGCTTGAGACAGGGGTCTG(+AGGACA)GATCCTCGGGAGACCACTGGGTATAATCTAT NGG 10 8 17 8 24 0 100 5 10
    240613154743_4kni GAAAAATAGATTATACCCAGTGGTCTCCCGAG(+AGGACA)GATCCAGACCCCTGTCTCAAGCTCCCTAACA NGG 10 8 17 8 24 0 100 5 10
    240613154741_8rjt TGTTTCATTTGAAGTATGGAGCTCTAAAAGGG(+AGGACA)GATCCTGTGAGAGGCCTCGGGTTACTAGATG NGG 10 8 17 8 24 0 100 5 10
    240613154740_flyc ACAAGCATCTAGTAACCCGAGGCCTCTCACAG(+AGGACA)GATCCCCTTTTAGAGCTCCATACTTCAAATG NGG 10 8 17 8 24 0 100 5 10
    240613154738_nwik AAAACTCATGCATAAAACAACAGGTTTCCTTG(+AGGACA)GATCCACAGCCTACCCTAAAATATGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613154737_4fxv CTATTTCTTTCCATATTTTAGGGTAGGCTGTG(+AGGACA)GATCCAAGGAAACCTGTTGTTTTATGCATGA NGG 10 8 17 8 24 0 100 5 10
    240613154735_fdlg CATCCTTGTCACTGGCACCCCTGCTGTGATGG(+AGGACA)GTACCTGGCAGCTTCCAAAATCCAAACTCTC NGG 10 8 17 8 24 0 100 5 10
    240613154733_d0dl TTGCAGAGAGTTTGGATTTTGGAAGCTGCCAG(+AGGACA)GTACCCATCACAGCAGGGGTGCCAGTGACAA NGG 10 8 17 8 24 0 100 5 10
    240613154732_npou CCTAAACAACAAAATTCATACATGAAAAAACG(+AGGACA)GTACCACAGGTTTTGGTGTCCTCACTAACAG NGG 10 8 17 8 24 0 100 5 10
    240613154730_fxor GACATCTGTTAGTGAGGACACCAAAACCTGTG(+AGGACA)GTACCGTTTTTTCATGTATGAATTTTGTTGT NGG 10 8 17 8 24 0 100 5 10