Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154638_aqhu TGAGGCCTGGCATTATTAGAGCATCTCACAGG(+AGGACA)GATCCTTTCTACATTAGATCAGAAGCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240613154637_wn4x GGACCCCTGGGCTTCTGATCTAATGTAGAAAG(+AGGACA)GATCCCTGTGAGATGCTCTAATAATGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240613154636_4c63 CTGCGAACCTACTCAGGGCTATTTGCTCTCTG(+AGGACA)GATCCCTGGTCTGCATTCACTCCATAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613154633_wx2o CCCACATTTTTATGGAGTGAATGCAGACCAGG(+AGGACA)GATCCAGAGAGCAAATAGCCCTGAGTAGGTT NGG 10 8 17 8 24 0 100 5 10
    240613154631_34tk CTACTGTAATCCCAGAAATTGGAATTCCTTGG(+AGGACA)GTACCCAAGTCCCCTGCTATAATTAGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240613154629_wlb3 ATTGTGCCTACTAATTATAGCAGGGGACTTGG(+AGGACA)GTACCCAAGGAATTCCAATTTCTGGGATTAC NGG 10 8 17 8 24 0 100 5 10
    240613154628_koio TGGACAAAGCGGGATGTGCGCAGAATCTAATG(+AGGACA)GATCCCGGCAAATCTTCCAGGTAGGAATATT NGG 10 8 17 8 24 0 100 5 10
    240613154626_ctnb GGAACAATATTCCTACCTGGAAGATTTGCCGG(+AGGACA)GATCCATTAGATTCTGCGCACATCCCGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613154624_ndcn AGGACTAGGGCAGAAGTCAAAAGCCTTCCTGG(+AGGACA)GTACCTGCAGACAAGCGGGCAAGGGTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613154622_wkhj TGCCAATCTCACCCTTGCCCGCTTGTCTGCAG(+AGGACA)GTACCCAGGAAGGCTTTTGACTTCTGCCCTA NGG 10 8 17 8 24 0 100 5 10