Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154536_avue CTGTCCATAGCCTGGCACCTCCTGAAGGACAG(+AGGACA)GATCCTGTGGGACGGGGCTGTGTTGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240613154534_mech GAGAGTGCAGACAACACAGCCCCGTCCCACAG(+AGGACA)GATCCTGTCCTTCAGGAGGTGCCAGGCTATG NGG 10 8 17 8 24 0 100 5 10
    240613154533_5mpk CACTTATTTCCATTGGGACTCAAGTGACCAAG(+AGGACA)GTACCCTTGAAAACTCTAAGCCTACAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613154531_df3j CACCTGAGGCTGTAGGCTTAGAGTTTTCAAGG(+AGGACA)GTACCTTGGTCACTTGAGTCCCAATGGAAAT NGG 10 8 17 8 24 0 100 5 10
    240613154529_u3ud CATAAACAAGGAAGCCCAATCAATTTCAGAGG(+AGGACA)GATCCCATTACTTAAGTCCTTAAAGGTTGGT NGG 10 8 17 8 24 0 100 5 10
    240613154528_93h6 CATGAACCAACCTTTAAGGACTTAAGTAATGG(+AGGACA)GATCCCTCTGAAATTGATTGGGCTTCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240613154526_dn6t GTTAAGGCTGGTCTGCTGGGCTACAAAGGGAG(+AGGACA)GTACCATTGCAGCCAACCCTTTACAGCTCTA NGG 10 8 17 8 24 0 100 5 10
    240613154525_a8o4 ACATCTAGAGCTGTAAAGGGTTGGCTGCAATG(+AGGACA)GTACCTCCCTTTGTAGCCCAGCAGACCAGCC NGG 10 8 17 8 24 0 100 5 10
    240613154524_guzo GCAGATGTGTGTGGCATGTGGCCAGTTCACAG(+AGGACA)GATCCTCCAGTTCTAAAACACACAGAGTACG NGG 10 8 17 8 24 0 100 5 10
    240613154522_ol0i CTGCCCGTACTCTGTGTGTTTTAGAACTGGAG(+AGGACA)GATCCTGTGAACTGGCCACATGCCACACACA NGG 10 8 17 8 24 0 100 5 10