Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154850_1dnm GTAGGGGTGGCTAACAAGCAGGAATAGGAAGG(+AGGACA)GATCCTTGGTAGCTTGTTTTTTGTCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240613154848_op3c AGATCCCAACAGACAAAAAACAAGCTACCAAG(+AGGACA)GATCCCTTCCTATTCCTGCTTGTTAGCCACC NGG 10 8 17 8 24 0 100 5 10
    240613154847_32db AAGTCACAGGTTGGTAGGTGTCATTGTCATTG(+AGGACA)GATCCTAGCTGACAGACAGATGAGGGGGAAT NGG 10 8 17 8 24 0 100 5 10
    240613154846_yh1n AGTGGATTCCCCCTCATCTGTCTGTCAGCTAG(+AGGACA)GATCCAATGACAATGACACCTACCAACCTGT NGG 10 8 17 8 24 0 100 5 10
    240613154844_2qgr AGCCTACCCCTGACACCGCCTGCAGGGCCAAG(+AGGACA)GATCCAAAGCCTGGAAAGGAGAACCAAACAT NGG 10 8 17 8 24 0 100 5 10
    240613154842_hg8a CAGGGATGTTTGGTTCTCCTTTCCAGGCTTTG(+AGGACA)GATCCTTGGCCCTGCAGGCGGTGTCAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240613154841_61nq GAATAATTTTTGGGACGTGGAAATTGCTAGAG(+AGGACA)GTACCTTTTCTGTAACAATGAGAAGAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613154840_hmjl TCCTAGAAGCTCTTCTCATTGTTACAGAAAAG(+AGGACA)GTACCTCTAGCAATTTCCACGTCCCAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613154838_wga3 GGCATTTTCCTAATTAGTGATTGATAGGGGAG(+AGGACA)GATCCAGCCATTTGTGGGTGGTGCCATCCCT NGG 10 8 17 8 24 0 100 5 10
    240613154837_ne44 AGCCAAGGGATGGCACCACCCACAAATGGCTG(+AGGACA)GATCCTCCCCTATCAATCACTAATTAGGAAA NGG 10 8 17 8 24 0 100 5 10