Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154939_ucrg GGGAGAGGATGACTCTCTTGATGGTGTATGGG(+AGGACA)GATCCACAACTTGAGGGTCATTTGGCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613154937_mosg CTATTGTGCTGCCAAATGACCCTCAAGTTGTG(+AGGACA)GATCCCCATACACCATCAAGAGAGTCATCCT NGG 10 8 17 8 24 0 100 5 10
    240613154935_g0bm ATGACTTCAGGCCTTTCCCAGCCTCCTCCCGG(+AGGACA)GATCCAGGAAGGCAGCAGGATATTTGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240613154933_wipp CCACATCACACAAATATCCTGCTGCCTTCCTG(+AGGACA)GATCCCGGGAGGAGGCTGGGAAAGGCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240613154932_2jpn GCAACTGACAGAAGTGTCAGCCTGGGCAGCAG(+AGGACA)GATCCCTCGGTTCTGTGGTGTCCCCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613154930_3iic CGTGTTGCCTGGGGGACACCACAGAACCGAGG(+AGGACA)GATCCTGCTGCCCAGGCTGACACTTCTGTCA NGG 10 8 17 8 24 0 100 5 10
    240613154928_0qfe GATTTGGAAGTGGGTGCACTGCCACACCTGTG(+AGGACA)GTACCAGCACTTGAGAAGCTGAGGCAGTAAG NGG 10 8 17 8 24 0 100 5 10
    240613154927_xx1n CCGGTCTTACTGCCTCAGCTTCTCAAGTGCTG(+AGGACA)GTACCACAGGTGTGGCAGTGCACCCACTTCC NGG 10 8 17 8 24 0 100 5 10
    240613154925_jwkr ATTAGATCTATGTAGATGTATCATCTTAAGGG(+AGGACA)GATCCTGCCGCCTCTGACAGCCATATCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613154923_brey ATAACAAGAGATATGGCTGTCAGAGGCGGCAG(+AGGACA)GATCCCCTTAAGATGATACATCTACATAGAT NGG 10 8 17 8 24 0 100 5 10