Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154956_r4ot GAAGTGCCTGTGGAGGTCAGACGAGGGTGTCG(+AGGACA)GATCCCCTGGATGTGGAGTTGCAGGCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613154953_f8lw GCTTACAACTGCCTGCAACTCCACATCCAGGG(+AGGACA)GATCCGACACCCTCGTCTGACCTCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240613154951_t3eg CAGTGAAGAGCACACTGGCTGCTCTCCCTGAG(+AGGACA)GATCCAAATTCAATTTTCAGCACCACATGGC NGG 10 8 17 8 24 0 100 5 10
    240613154950_xxaq AAGCTGCCATGTGGTGCTGAAAATTGAATTTG(+AGGACA)GATCCTCAGGGAGAGCAGCCAGTGTGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613154948_0gfl CAGTTCGCTCCCCATGCAGCCATACCAGATAG(+AGGACA)GTACCCTGGCTGGCTGTTAGAGAAGAGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613154946_2kar CTGGGGAAACTCTTCTCTAACAGCCAGCCAGG(+AGGACA)GTACCTATCTGGTATGGCTGCATGGGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240613154944_v1i7 CCAGAATCATATGACTGAGTTGGTAGAGCCAG(+AGGACA)GTACCAGTGTGTGATCTTAAAAGCAAGCCAT NGG 10 8 17 8 24 0 100 5 10
    240613154943_035v GCAGCATGGCTTGCTTTTAAGATCACACACTG(+AGGACA)GTACCTGGCTCTACCAACTCAGTCATATGAT NGG 10 8 17 8 24 0 100 5 10
    240613154942_yhuw AGCATTATACCCAATTGATTACACTAAATCAG(+AGGACA)GTACCAAAGTACTAGCTTTTCTGAAGGAGTC NGG 10 8 17 8 24 0 100 5 10
    240613154941_q1j2 ATTAAGACTCCTTCAGAAAAGCTAGTACTTTG(+AGGACA)GTACCTGATTTAGTGTAATCAATTGGGTATA NGG 10 8 17 8 24 0 100 5 10