Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155121_gnp4 TGATACCAAGACCTAACAAACCCCGTGCCTGG(+AGGACA)GTACCTCTTTTAAAGCTCTGCACCCCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240613155119_y34p CCTTGAAGAAGGGGTGCAGAGCTTTAAAAGAG(+AGGACA)GTACCCAGGCACGGGGTTTGTTAGGTCTTGG NGG 10 8 17 8 24 0 100 5 10
    240613155118_17jq ACAGCCCATCTGCCACAAGAGCTTCCTGATTG(+AGGACA)GTACCACTGTGAACCGTCCCTCCCCCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613155117_ogtb CTGTCTGGAGGGGGGAGGGACGGTTCACAGTG(+AGGACA)GTACCAATCAGGAAGCTCTTGTGGCAGATGG NGG 10 8 17 8 24 0 100 5 10
    240613155116_cdmp GTGCTTCAGGGTGGGCCCTATTTGGGCTTGAG(+AGGACA)GATCCCGTGGGGGCGGGGCTTCACCCCGACT NGG 10 8 17 8 24 0 100 5 10
    240613155114_hydl CACCCAGTCGGGGTGAAGCCCCGCCCCCACGG(+AGGACA)GATCCTCAAGCCCAAATAGGGCCCACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613155112_253u CTCTAGGAATCTCTATGGGGACTGCTAGGAAG(+AGGACA)GATCCTGGGCATGACAGCCTCGTATGATAGC NGG 10 8 17 8 24 0 100 5 10
    240613155110_g61h TGCAGGCTATCATACGAGGCTGTCATGCCCAG(+AGGACA)GATCCTTCCTAGCAGTCCCCATAGAGATTCC NGG 10 8 17 8 24 0 100 5 10
    240613155108_5waf ATAGATCCTAAGATCCAGCAAGCTTGGCTCAG(+AGGACA)GATCCTGTTGGGGAGCAGCCACCTGCCTTAT NGG 10 8 17 8 24 0 100 5 10
    240613155107_j7or CTGGGATAAGGCAGGTGGCTGCTCCCCAACAG(+AGGACA)GATCCTGAGCCAAGCTTGCTGGATCTTAGGA NGG 10 8 17 8 24 0 100 5 10