Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604190353_r492 TTCCACTCAAATCAGTTGAGAGCCCAAATCAG(+ACTCAC)GATCCACATATCATTCATTGTCATTAGATCT NGG 10 8 17 8 24 0 100 5 10
    240604190352_q72z TATTGAGATCTAATGACAATGAATGATATGTG(+ACTCAC)GATCCTGATTTGGGCTCTCAACTGATTTGAG NGG 10 8 17 8 24 0 100 5 10
    240604190350_fz8a GCTGTTAACAGGAGGTCCAAACCTCTTGGAAG(+ACTCAC)GTACCAAGCAGCTAATAAAGGCTGGTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604190348_uv6y GATGAAGAAGACCAGCCTTTATTAGCTGCTTG(+ACTCAC)GTACCTTCCAAGAGGTTTGGACCTCCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240604190347_9rdt AGTCTCCCTAGCGCCACTGTGCTGAGAACAGG(+ACTCAC)GATCCTCTGGATGAGGAAGCCCTGGCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604190345_wmaz CTGAGCTGGGGCCAGGGCTTCCTCATCCAGAG(+ACTCAC)GATCCCTGTTCTCAGCACAGTGGCGCTAGGG NGG 10 8 17 8 24 0 100 5 10
    240604190343_xz9r CATCTAACAGCAGCTTGAACCTTGGATCTTAG(+ACTCAC)GATCCGCTTGACATAGTCTGCTTTCTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240604190342_mrbc AAGTTCTCCGAGAAAGCAGACTATGTCAAGCG(+ACTCAC)GATCCTAAGATCCAAGGTTCAAGCTGCTGTT NGG 10 8 17 8 24 0 100 5 10
    240604190340_gn2i AGTGTCTGGGTTTGGTGACTGATTATGGGATG(+ACTCAC)GATCCGGGTGGGGTATGCTCTGGATAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240604190338_jz82 AAGGATGAACTATCCAGAGCATACCCCACCCG(+ACTCAC)GATCCATCCCATAATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10