Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604190335_r0mm TCTTTCTCTAGCTCTTCCATTGGGGGACCTGG(+ACTCAC)GATCCATCCAATAGCTGACTGTGAGCATCCA NGG 10 8 17 8 24 0 100 5 10
    240604190333_1f5p AGAAGTGGATGCTCACAGTCAGCTATTGGATG(+ACTCAC)GATCCCAGGTCCCCCAATGGAAGAGCTAGAG NGG 10 8 17 8 24 0 100 5 10
    240604190332_y1r3 CTAATGGTGCGTTTGTTTCTGCTTTTGACTTG(+ACTCAC)GTACCTGTGCACTCTAAGCAGGTAGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240604190330_hap6 TTCCCCAGGGCCTACCTGCTTAGAGTGCACAG(+ACTCAC)GTACCAAGTCAAAAGCAGAAACAAACGCACC NGG 10 8 17 8 24 0 100 5 10
    240604190328_8pf6 GACAGCTCCTTCAGGCAGTAGCTGTGAAGGAG(+ACTCAC)GTACCTGTGTCTCAGAAATGGCGTGTCCACA NGG 10 8 17 8 24 0 100 5 10
    240604190327_yweu AAACATGTGGACACGCCATTTCTGAGACACAG(+ACTCAC)GTACCTCCTTCACAGCTACTGCCTGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240604190326_kopt ACCAAGGGGGGAAATCCCAGCAGATAATTTAG(+ACTCAC)GATCCCCTTCTAAACACATGAGGGCCTGTAT NGG 10 8 17 8 24 0 100 5 10
    240604190323_7avu TGCACATACAGGCCCTCATGTGTTTAGAAGGG(+ACTCAC)GATCCTAAATTATCTGCTGGGATTTCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240604190322_xo7y AATGTTTAGAGAACAATTAGGTCAATTTCAGG(+ACTCAC)GATCCACTTGGTGTATGTGTGGACCTACAGA NGG 10 8 17 8 24 0 100 5 10
    240604190320_vvot ACCTATCTGTAGGTCCACACATACACCAAGTG(+ACTCAC)GATCCCTGAAATTGACCTAATTGTTCTCTAA NGG 10 8 17 8 24 0 100 5 10