Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604190238_jk1i ATAAAGGTTCAGGTGACAGGAACAGAAATCCG(+ACTCAC)GTACCCCAGGGGCTCTAAATAACACCGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604190236_p5on ACTGCTCCACGGTGTTATTTAGAGCCCCTGGG(+ACTCAC)GTACCGGATTTCTGTTCCTGTCACCTGAACC NGG 10 8 17 8 24 0 100 5 10
    240604190234_wzwr TGTGGCTGGCATTTACATGGATGTGAGGGGAG(+ACTCAC)GATCCGAACTCAAATCTTCACATATGGATGG NGG 10 8 17 8 24 0 100 5 10
    240604190232_ejq5 GCTTGCCATCCATATGTGAAGATTTGAGTTCG(+ACTCAC)GATCCTCCCCTCACATCCATGTAAATGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604190230_3kg7 CTACCGACAGTAAAAGTTAAAAGCAACACCAG(+ACTCAC)GTACCTGCATCCATTTACAATACTTGCAAAT NGG 10 8 17 8 24 0 100 5 10
    240604190229_y2b6 CTGTGATTTGCAAGTATTGTAAATGGATGCAG(+ACTCAC)GTACCTGGTGTTGCTTTTAACTTTTACTGTC NGG 10 8 17 8 24 0 100 5 10
    240604190226_l3ag CAATTTTACAAATAAATACTTTCTTTCCTTGG(+ACTCAC)GTACCTAGAATAATTAATCCAGCATACAACT NGG 10 8 17 8 24 0 100 5 10
    240604190225_vfw3 TTCAAAGTTGTATGCTGGATTAATTATTCTAG(+ACTCAC)GTACCCAAGGAAAGAAAGTATTTATTTGTAA NGG 10 8 17 8 24 0 100 5 10
    240604190223_dloa TAAATGGGCTTCTCAGATAGTACCCTCCACAG(+ACTCAC)GTACCCCCTTAATCAATACCATGCAAATTGT NGG 10 8 17 8 24 0 100 5 10
    240604190220_5egz TGAGCACAATTTGCATGGTATTGATTAAGGGG(+ACTCAC)GTACCTGTGGAGGGTACTATCTGAGAAGCCC NGG 10 8 17 8 24 0 100 5 10