Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604185440_xmio TTACTCTTAATTACTGTGCTCACAAAGCCTCG(+ACTCAC)GATCCTTGGATCTCTTGCATCTGTGACTAAC NGG 10 8 17 8 24 0 100 5 10
    240604185439_0xj8 CTTAGAATTCTCAATGGAAGAGAATGGTACAG(+ACTCAC)GTACCAACAACATGCCATATGCATAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240604185437_3mxg CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+ACTCAC)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240604185436_w8kz ATGTAATAGTGTCCAGCCAAACAATCCAACAG(+ACTCAC)GATCCTCCACTCTTCTTTCATGTAAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240604185434_z4qi TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCAC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    240604185132_3z4i CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+ACTCAC)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240604185120_ckay CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+ACTCAC)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240604184432_3xjq CTTAGAATTCTCAATGGAAGAGAATGGTACAG(+ACTCAC)GTACCAACAACATGCCATATGCATAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240604183954_4k16 CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+ACTCAC)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240604183811_9oh5 ATAAAGGTTCAGGTGACAGGAACAGAAATCCG(+ACTCAC)GTACCCCAGGGGCTCTAAATAACACCGTGGA NGG 10 8 17 8 24 0 100 5 10