Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604183604_giud ACTGCGATTCTCCAATGGGTTACGTGCCAATG(+ACTCAC)GTACCTACAATGTATTGTTATAAGACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240604183600_tdde TCCATTCTAGGTCTTATAACAATACATTGTAG(+ACTCAC)GTACCATTGGCACGTAACCCATTGGAGAATC NGG 10 8 17 8 24 0 100 5 10
    240604183556_7lr6 CTGTGGTTAGTCACAGATGCAAGAGATCCAAG(+ACTCAC)GATCCGAGGCTTTGTGAGCACAGTAATTAAG NGG 10 8 17 8 24 0 100 5 10
    240604183552_t7vy TTACTCTTAATTACTGTGCTCACAAAGCCTCG(+ACTCAC)GATCCTTGGATCTCTTGCATCTGTGACTAAC NGG 10 8 17 8 24 0 100 5 10
    240604183549_zcz3 CTTAGAATTCTCAATGGAAGAGAATGGTACAG(+ACTCAC)GTACCAACAACATGCCATATGCATAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240604183546_plu4 CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+ACTCAC)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240604183543_zexm ATGTAATAGTGTCCAGCCAAACAATCCAACAG(+ACTCAC)GATCCTCCACTCTTCTTTCATGTAAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240604183539_4b2a TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCAC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    240604183429_fopt ATAAAGGTTCAGGTGACAGGAACAGAAATCCG(+ACTCAC)GTACCCCAGGGGCTCTAAATAACACCGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604183424_si7v ACTGCTCCACGGTGTTATTTAGAGCCCCTGGG(+ACTCAC)GTACCGGATTTCTGTTCCTGTCACCTGAACC NGG 10 8 17 8 24 0 100 5 10