Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604183000_e46i ACTGCGATTCTCCAATGGGTTACGTGCCAATG(+ACTCAC)GTACCTACAATGTATTGTTATAAGACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240604182957_d1t4 TCCATTCTAGGTCTTATAACAATACATTGTAG(+ACTCAC)GTACCATTGGCACGTAACCCATTGGAGAATC NGG 10 8 17 8 24 0 100 5 10
    240604182953_ee6q CTGTGGTTAGTCACAGATGCAAGAGATCCAAG(+ACTCAC)GATCCGAGGCTTTGTGAGCACAGTAATTAAG NGG 10 8 17 8 24 0 100 5 10
    240604182949_qn40 TTACTCTTAATTACTGTGCTCACAAAGCCTCG(+ACTCAC)GATCCTTGGATCTCTTGCATCTGTGACTAAC NGG 10 8 17 8 24 0 100 5 10
    240604182946_e3ul CTTAGAATTCTCAATGGAAGAGAATGGTACAG(+ACTCAC)GTACCAACAACATGCCATATGCATAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240604182942_wh85 CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+ACTCAC)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240604182939_fgfj ATGTAATAGTGTCCAGCCAAACAATCCAACAG(+ACTCAC)GATCCTCCACTCTTCTTTCATGTAAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240604182936_t3m3 TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCAC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    240604182849_9c27 ATAAAGGTTCAGGTGACAGGAACAGAAATCCG(+ACTCAC)GTACCCCAGGGGCTCTAAATAACACCGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604182845_73p9 ACTGCTCCACGGTGTTATTTAGAGCCCCTGGG(+ACTCAC)GTACCGGATTTCTGTTCCTGTCACCTGAACC NGG 10 8 17 8 24 0 100 5 10