Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604182841_76yh TGTGGCTGGCATTTACATGGATGTGAGGGGAG(+ACTCAC)GATCCGAACTCAAATCTTCACATATGGATGG NGG 10 8 17 8 24 0 100 5 10
    240604182838_t0ai GCTTGCCATCCATATGTGAAGATTTGAGTTCG(+ACTCAC)GATCCTCCCCTCACATCCATGTAAATGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604182834_v95k CTACCGACAGTAAAAGTTAAAAGCAACACCAG(+ACTCAC)GTACCTGCATCCATTTACAATACTTGCAAAT NGG 10 8 17 8 24 0 100 5 10
    240604182830_5sbt CTGTGATTTGCAAGTATTGTAAATGGATGCAG(+ACTCAC)GTACCTGGTGTTGCTTTTAACTTTTACTGTC NGG 10 8 17 8 24 0 100 5 10
    240604182826_8egz CAATTTTACAAATAAATACTTTCTTTCCTTGG(+ACTCAC)GTACCTAGAATAATTAATCCAGCATACAACT NGG 10 8 17 8 24 0 100 5 10
    240604182823_ppcm TTCAAAGTTGTATGCTGGATTAATTATTCTAG(+ACTCAC)GTACCCAAGGAAAGAAAGTATTTATTTGTAA NGG 10 8 17 8 24 0 100 5 10
    240604182819_rdxy TAAATGGGCTTCTCAGATAGTACCCTCCACAG(+ACTCAC)GTACCCCCTTAATCAATACCATGCAAATTGT NGG 10 8 17 8 24 0 100 5 10
    240604182814_iugi TGAGCACAATTTGCATGGTATTGATTAAGGGG(+ACTCAC)GTACCTGTGGAGGGTACTATCTGAGAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240604182811_bq4q TAAGCCTTCCTTCATTCTGAATACTTCACTTG(+ACTCAC)GTACCATGCTAGGACTGAGAGTCATATTTCA NGG 10 8 17 8 24 0 100 5 10
    240604182807_ywsj CTTTGTGAAATATGACTCTCAGTCCTAGCATG(+ACTCAC)GTACCAAGTGAAGTATTCAGAATGAAGGAAG NGG 10 8 17 8 24 0 100 5 10