Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604182618_1d3k ATGTAATAGTGTCCAGCCAAACAATCCAACAG(+ACTCAC)GATCCTCCACTCTTCTTTCATGTAAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240604182614_wrkc TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCAC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    240604182602_rzk0 CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+ACTCAC)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240604182559_au5v ATGTAATAGTGTCCAGCCAAACAATCCAACAG(+ACTCAC)GATCCTCCACTCTTCTTTCATGTAAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240604182555_4k5c TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCAC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    240604182202_bv54 CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+ACTCAC)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240604181911_j4ob ATAAAGGTTCAGGTGACAGGAACAGAAATCCG(+ACTCAC)GTACCCCAGGGGCTCTAAATAACACCGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604181906_4zzt ACTGCTCCACGGTGTTATTTAGAGCCCCTGGG(+ACTCAC)GTACCGGATTTCTGTTCCTGTCACCTGAACC NGG 10 8 17 8 24 0 100 5 10
    240604181902_za14 TGTGGCTGGCATTTACATGGATGTGAGGGGAG(+ACTCAC)GATCCGAACTCAAATCTTCACATATGGATGG NGG 10 8 17 8 24 0 100 5 10
    240604181859_ece3 GCTTGCCATCCATATGTGAAGATTTGAGTTCG(+ACTCAC)GATCCTCCCCTCACATCCATGTAAATGCCAG NGG 10 8 17 8 24 0 100 5 10