Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604181855_cjda CTACCGACAGTAAAAGTTAAAAGCAACACCAG(+ACTCAC)GTACCTGCATCCATTTACAATACTTGCAAAT NGG 10 8 17 8 24 0 100 5 10
    240604181852_8g2y CTGTGATTTGCAAGTATTGTAAATGGATGCAG(+ACTCAC)GTACCTGGTGTTGCTTTTAACTTTTACTGTC NGG 10 8 17 8 24 0 100 5 10
    240604181848_w0g8 CAATTTTACAAATAAATACTTTCTTTCCTTGG(+ACTCAC)GTACCTAGAATAATTAATCCAGCATACAACT NGG 10 8 17 8 24 0 100 5 10
    240604181845_cpww TTCAAAGTTGTATGCTGGATTAATTATTCTAG(+ACTCAC)GTACCCAAGGAAAGAAAGTATTTATTTGTAA NGG 10 8 17 8 24 0 100 5 10
    240604181841_764k TAAATGGGCTTCTCAGATAGTACCCTCCACAG(+ACTCAC)GTACCCCCTTAATCAATACCATGCAAATTGT NGG 10 8 17 8 24 0 100 5 10
    240604181837_nyvx TGAGCACAATTTGCATGGTATTGATTAAGGGG(+ACTCAC)GTACCTGTGGAGGGTACTATCTGAGAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240604181833_laen TAAGCCTTCCTTCATTCTGAATACTTCACTTG(+ACTCAC)GTACCATGCTAGGACTGAGAGTCATATTTCA NGG 10 8 17 8 24 0 100 5 10
    240604181830_zrf7 CTTTGTGAAATATGACTCTCAGTCCTAGCATG(+ACTCAC)GTACCAAGTGAAGTATTCAGAATGAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604181826_m9tn ACTGCGATTCTCCAATGGGTTACGTGCCAATG(+ACTCAC)GTACCTACAATGTATTGTTATAAGACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240604181823_i4s9 TCCATTCTAGGTCTTATAACAATACATTGTAG(+ACTCAC)GTACCATTGGCACGTAACCCATTGGAGAATC NGG 10 8 17 8 24 0 100 5 10