Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604151314_lcu7 TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCAC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    240604142316_l0z1 TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCAC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    240604141206_z68v GCCCACTGAAGGGCATCTTGGGCTACACTGAGGACCAGGTTGTCTCCTGCGACTTCAACAGCAACTCCCACTCTTCCACCTTCGATGCCGGGGCTGGCATTGCTCTCAATGACAACTTTGTCAAGCTCATTTCCTGGTATGTGGGGATGGGAAACCTGACTTTTAAGAGCAACTGGGGGTTTGGTGCCCTCTGGTGGCTAGCTCAGAAAAGAAACCCAAACTAACAGTTGTCCCAATTTGTTCTAGGTATGACAATGAATACGGCTACAGCAACAGGGTGGTGGACCTCATGGCCTACATGGCCTCCAAGGAG(+ctt)TAAGAAACCCTGGACCACCCACCCCAGCAAGGACACTGAGCAAGAGAGGCCCTATCCCAACTCGGCCCCCAACACTGAGCATCTCCCTCACAATTTCCATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAA NGG 10 8 17 8 24 0 100 5 10
    240604135952_z9gp GCCCACTGAAGGGCATCTTGGGCTACACTGAGGACCAGGTTGTCTCCTGCGACTTCAACAGCAACTCCCACTCTTCCACCTTCGATGCCGGGGCTGGCATTGCTCTCAATGACAACTTTGTCAAGCTCATTTCCTGGTATGTGGGGATGGGAAACCTGACTTTTAAGAGCAACTGGGGGTTTGGTGCCCTCTGGTGGCTAGCTCAGAAAAGAAACCCAAACTAACAGTTGTCCCAATTTGTTCTAGGTATGACAATGAATACGGCTACAGCAACAGGGTGGTGGACCTCATGGCCTACATGGCCTCCAAG(/A)GAGTAAGAAACCCTGGACCACCCACCCCAGCAAGGACACTGAGCAAGAGAGGCCCTATCCCAACTCGGCCCCCAACACTGAGCATCTCCCTCACAATTTCCATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAA NGG 10 8 17 8 24 0 100 5 10
    240604135529_ta4p GCCCACTGAAGGGCATCTTGGGCTACACTGAGGACCAGGTTGTCTCCTGCGACTTCAACAGCAACTCCCACTCTTCCACCTTCGATGCCGGGGCTGGCATTGCTCTCAATGACAACTTTGTCAAGCTCATTTCCTGGTATGTGGGGATGGGAAACCTGACTTTTAAGAGCAACTGGGGGTTTGGTGCCCTCTGGTGGCTAGCTCAGAAAAGAAACCCAAACTAACAGTTGTCCCAATTTGTTCTAGGTATGACAATGAATACGGCTACAGCAACAGGGTGGTGGACCTCATGGCCTACATGGCCTCC(/A)AAGGAGTAAGAAACCCTGGACCACCCACCCCAGCAAGGACACTGAGCAAGAGAGGCCCTATCCCAACTCGGCCCCCAACACTGAGCATCTCCCTCACAATTTCCATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAA NGG 10 8 17 8 24 0 100 5 10
    240604133037_syx3 GCCCACTGAAGGGCATCTTGGGCTACACTGAGGACCAGGTTGTCTCCTGCGACTTCAACAGCAACTCCCACTCTTCCACCTTCGATGCCGGGGCTGGCATTGCTCTCAATGACAACTTTGTCAAGCTCATTTCCTGGTATGTGGGGATGGGAAACCTGACTTTTAAGAGCAACTGGGGGTTTGGTGCCCTCTGGTGGCTAGCTCAGAAAAGAAACCCAAACTAACAGTTGTCCCAATTTGTTCTAGGTATGACAATGAATACGGCTACAGCAACAGGGTGGTGGACCTCATGGCCTACATGGCCTCCAAGGAGTAAGAAACCCTGGACCACCCACCCCAGCAAGGACACTGAGCAAGAGAGGCCCTATC(+ctt)CCAACTCGGCCCCCAACACTGAGCATCTCCCTCACAATTTCCATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCACCCACCTCTCCTTGGTTTTAATGCGGTGTTGGGGGAGGGAGGGTGTGTATGGGGAGGTGAAGCAGGCTCAATCAACTTTTGTGCATGTATCTTTATTGGCTCTGACATCCAGCTGGGTGCCGGAAGTCCAGGGCTACATTCTATCCAAG NGG 10 8 17 8 24 0 100 5 10
    240604132532_g71e GCCCACTGAAGGGCATCTTGGGCTACACTGAGGACCAGGTTGTCTCCTGCGACTTCAACAGCAACTCCCACTCTTCCACCTTCGATGCCGGGGCTGGCATTGCTCTCAATGACAACTTTGTCAAGCTCATTTCCTGGTATGTGGGGATGGGAAACCTGACTTTTAAGAGCAACTGGGGGTTTGGTGCCCTCTGGTGGCTAGCTCAGAAAAGAAACCCAAACTAACAGTTGTCCCAATTTGTTCTAGGTATGACAATGAATACGGCTACAGCAACAGGGTGGTGGACCTCATGGCCTACATGGCCT(+gga)CCAAGGAGTAAGAAACCCTGGACCACCCACCCCAGCAAGGACACTGAGCAAGAGAGGCCCTATCCCAACTCGGCCCCCAACACTGAGCATCTCCCTCACAATTTCCATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAA NGG 10 8 17 8 24 0 100 5 10
    240604131748_l8a6 GCCCACTGAAGGGCATCTTGGGCTACACTGAGGACCAGGTTGTCTCCTGCGACTTCAACAGCAACTCCCACTCTTCCACCTTCGATGCCGGGGCTGGCATTGCTCTCAATGACAACTTTGTCAAGCTCATTTCCTGGTATGTGGGGATGGGAAACCTGACTTTTAAGAGCAACTGGGGGTTTGGTGCCCTCTGGTGGCTAGCTCAGAAAAGAAACCCAAACTAACAGTTGTCCCAATTTGTTCTAGGTATGACAATGAATACGGCTACAGCAACAGGGTGGTGGACCTCATGGCCTACATGGCCTCCAAGGAGTAAGAAACCCTGGACCACCCACCCCAGCAAGGACACTGAGCAAGAGAGGCCCTATC(+ctt)CCAACTCGGCCCCCAACACTGAGCATCTCCCTCACAATTTCCATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCACCCACCTCTCCTTGGTTTTAATGCGGTGTTGGGGGAGGGAGGGTGTGTATGGGGAGGTGAAGCAGGCTCAATCAACTTTTGTGCATGTATCTTTATTGGCTCTGACATCCAGCTGGGTGCCGGAAGTCCAGGGCTACATTCTATCCAAG NGG 10 8 17 8 24 0 100 5 10
    240604131631_e4vt GCCCACTGAAGGGCATCTTGGGCTACACTGAGGACCAGGTTGTCTCCTGCGACTTCAACAGCAACTCCCACTCTTCCACCTTCGATGCCGGGGCTGGCATTGCTCTCAATGACAACTTTGTCAAGCTCATTTCCTGGTATGTGGGGATGGGAAACCTGACTTTTAAGAGCAACTGGGGGTTTGGTGCCCTCTGGTGGCTAGCTCAGAAAAGAAACCCAAACTAACAGTTGTCCCAATTTGTTCTAGGTATGACAATGAATACGGCTACAGCAACAGGGTGGTGGACCTCATGGCCTACATGGCCTCCAAGGAGTAAGAAACCCTGGACCACCCACCCCAGCAAGGACACTGAGCAAGAGAGGCCCTATCCCAACTCGGCCCCCAACACTGAGCATCTCCCT(+ctt)CACAATTTCCATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCACCCACCTCTCCTTGGTTTTAATGCGGTGTTGGGGGAGGGAGGGTGTGTATGGGGAGGTGAAGCAGGCTCAATCAACTTTTGTGCATGTATCTTTATTGGCTCTGACATCCAGCTGGGTGCCGGAAGTCCAGGGCTACATTCTATCCAAG NGG 10 8 17 8 24 0 100 5 10
    240604131515_iyr6 GCCCACTGAAGGGCATCTTGGGCTACACTGAGGACCAGGTTGTCTCCTGCGACTTCAACAGCAACTCCCACTCTTCCACCTTCGATGCCGGGGCTGGCATTGCTCTCAATGACAACTTTGTCAAGCTCATTTCCTGGTATGTGGGGATGGGAAACCTGACTTTTAAGAGCAACTGGGGGTTTGGTGCCCTCTGGTGGCTAGCTCAGAAAAGAAACCCAAACTAACAGTTGTCCCAATTTGTTCTAGGTATGACAATGAATACGGCTACAGCAACAGGGTGGTGGACCTCATGGCCTACATGGCCT(+ctt)CCAAGGAGTAAGAAACCCTGGACCACCCACCCCAGCAAGGACACTGAGCAAGAGAGGCCCTATCCCAACTCGGCCCCCAACACTGAGCATCTCCCTCACAATTTCCATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAA NGG 10 8 17 8 24 0 100 5 10