Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604103315_jbct ACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGTTGAGGCAGCCAGGGCTGGCCTGTACACTGACTTGAGACCAATAAAAGTGCACACCTTAC(+ctt)CTTACACAAACAGCTTGTGGCTCTGTGGCTTTGCTGGGTGTGGGGAGCAGGTTGGGTGGGTGTGGAGCTCTATTGGGGGGGGCATCTAGGGTGGGCTAGGCCTTGCTGATGGTATCTAGTGGGAGGGCTACAGGCCCTTTAATGAAGTCTCACAAATAACCACACCTTCAGACTGTCTTGAAGAGTTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604103248_b4f9 ACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGTTGAGGCAGCCAGGGCTGGCCTGTACACTGACTTGAGACCAATAAAAGTGCACACCTTACCTTAC(+ctt)ACAAACAGCTTGTGGCTCTGTGGCTTTGCTGGGTGTGGGGAGCAGGTTGGGTGGGTGTGGAGCTCTATTGGGGGGGGCATCTAGGGTGGGCTAGGCCTTGCTGATGGTATCTAGTGGGAGGGCTACAGGCCCTTTAATGAAGTCTCACAAATAACCACACCTTCAGACTGTCTTGAAGAGTTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604102928_cpbq CTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGTTGAGGCAGCCAGGGCTGGCCTGTACACTGACTTGAGACCAATAAAAGTGCACACCTTACCTTACACAAACAGCTTGTGGCTCTGTGGCTTTGCTGGGTGTGGGGAGCAGGTTGGGTGGGTGTGGAGCTCTATTGGGGGGGGCATCTAGGGTGGGCTAGGCCTTGC(+ctt)TGATGGTATCTAGTGGGAGGGCTACAGGCCCTTTAATGAAGTCTCACAAATAACCACACCTTCAGACTGTCTTGAAGAGTTGAAAAGGTACTCCTCCAGAAACCTAGGCCAAGTTGGCCTGCACTAACACTACCTTCCTCAACCGAGGGGTGTCCACACCCTTTGCCTCCATCTGCATAACTGGGTTTCAAGCCCCTCTGCTAGTCTGAAGCTGCCCTTTCCAGAGCTGTGACTGACAGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604102755_o5ih ACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGTTGAGGCAGCCAGGGCTGGCCTGTACACTGACTTGAGACCAATAAAAGTGCACACCTTAC(+ctt)CTTACACAAACAGCTTGTGGCTCTGTGGCTTTGCTGGGTGTGGGGAGCAGGTTGGGTGGGTGTGGAGCTCTATTGGGGGGGGCATCTAGGGTGGGCTAGGCCTTGCTGATGGTATCTAGTGGGAGGGCTACAGGCCCTTTAATGAAGTCTCACAAATAACCACACCTTCAGACTGTCTTGAAGAGTTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604102729_lq14 ACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGTTGAGGCAGCCAGGGCTGGCCTGTACACTGACTTGAGACCAATAAAAGTGCACACCTTACCTTAC(+ctt)ACAAACAGCTTGTGGCTCTGTGGCTTTGCTGGGTGTGGGGAGCAGGTTGGGTGGGTGTGGAGCTCTATTGGGGGGGGCATCTAGGGTGGGCTAGGCCTTGCTGATGGTATCTAGTGGGAGGGCTACAGGCCCTTTAATGAAGTCTCACAAATAACCACACCTTCAGACTGTCTTGAAGAGTTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604102347_jj9m ACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGTTGAGGCAGCCAG(+ctt)GGCTGGCCTGTACACTGACTTGAGACCAATAAAAGTGCACACCTTACCTTACACAAACAGCTTGTGGCTCTGTGGCTTTGCTGGGTGTGGGGAGCAGGTTGGGTGGGTGTGGAGCTCTATTGGGGGGGGCATCTAGGGTGGGCTAGGCCTTGCTGATGGTATCTAGTGGGAGGGCTACAGGCCCTTTAATGAAGTCTCACAAATAACCACACCTTCAGACTGTCTTGAAGAGTTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604101711_7ltx TTCTCTCTCGCTTTTTTTTTTTTTCTTCTTCTTTTTTTTTTTTTTTTTCAAAAGGAGGGGAGAGGGGGTAAAAAAATGCTGCACTGTGCGGCGAGGCCGGTGAGTGAGCGACGCGGAGCCAATCAGCGCCCGCCGTTCCGAAAGTTGCCTTTTATGGCTCGAGTGGCCGCTGTGGCGTCCTATAAAACCCGGCGGCGCAACGCGCAGCCACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCCTTCGTTGCCGGTCCACACCCGCCACCAGGT(+ctt)AAGCAGGGACGCCGGGCCCAGCGGGCCTTCGCTCTCTCGTGGCTAGTACCTCACTGCAGGGTCCTGAGGATCACTCAGAACGGACACCATGGGCGGGTGGAGGGTGGTGCCGGGCCGCGGAGCGGACACTGGCACAGCCAACTTTACGCCTAGCGTGTAGACTCTTTGCAGCCACATTCCCGCGGTGTAGACACT NGG 10 8 17 8 24 0 100 5 10
    240604101637_z47z TTCTCTCTCGCTTTTTTTTTTTTTCTTCTTCTTTTTTTTTTTTTTTTTCAAAAGGAGGGGAGAGGGGGTAAAAAAATGCTGCACTGTGCGGCGAGGCCGGTGAGTGAGCGACGCGGAGCCAATCAGCGCCCGCCGTTCCGAAAGTTGCCTTTTATGGCTCGAGTGGCCGCTGTGGCGTCCTATAAAACCCGGCGGCGCAACGCGCAGCCACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCCTTCG(+ctt)TTGCCGGTCCACACCCGCCACCAGGTAAGCAGGGACGCCGGGCCCAGCGGGCCTTCGCTCTCTCGTGGCTAGTACCTCACTGCAGGGTCCTGAGGATCACTCAGAACGGACACCATGGGCGGGTGGAGGGTGGTGCCGGGCCGCGGAGCGGACACTGGCACAGCCAACTTTACGCCTAGCGTGTAGACTCTTTGCAGCCACATTCCCGCGGTGTAGACACT NGG 10 8 17 8 24 0 100 5 10
    240604101506_phlg TTCTCTCTCGCTTTTTTTTTTTTTCTTCTTCTTTTTTTTTTTTTTTTTCAAAAGGAGGGGAGAGGGGGTAAAAAAATGCTGCACTGTGCGGCGAGGCCGGTGAGTGAGCGACGCGGAGCCAATCAGCGCCCGCCGTTCCGAAAGTTGCCTTTTATGGCTCGAGTGGCCGCTGTGGCGTCCTATAAAACCCGGCGGCGCAACGCGCAGCCACTGTCGAGTCGCGTCCACCC(+ctt)GCGAGCACAGCTTCTTTGCAGCTCCTTCGTTGCCGGTCCACACCCGCCACCAGGTAAGCAGGGACGCCGGGCCCAGCGGGCCTTCGCTCTCTCGTGGCTAGTACCTCACTGCAGGGTCCTGAGGATCACTCAGAACGGACACCATGGGCGGGTGGAGGGTGGTGCCGGGCCGCGGAGCGGACACTGGCACAGCCAACTTTACGCCTAGCGTGTAGACTCTTTGCAGCCACATTCCCGCGGTGTAGACACT NGG 10 8 17 8 24 0 100 5 10
    240604101422_3kxx TTCTCTCTCGCTTTTTTTTTTTTTCTTCTTCTTTTTTTTTTTTTTTTTCAAAAGGAGGGGAGAGGGGGTAAAAAAATGCTGCACTGTGCGGCGAGGCCGGTGAGTGAGCGACGCGGAGCCAATCAGCGCCCGCCGTTCCGAAAGTTGCCTTTTATGGCTCGAGTGGCCGCTGTGGCGTCCTATAAAACCCGGCGGCGCAACGCGCAGCCACTG(+ctt)TCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCCTTCGTTGCCGGTCCACACCCGCCACCAGGTAAGCAGGGACGCCGGGCCCAGCGGGCCTTCGCTCTCTCGTGGCTAGTACCTCACTGCAGGGTCCTGAGGATCACTCAGAACGGACACCATGGGCGGGTGGAGGGTGGTGCCGGGCCGCGGAGCGGACACTGGCACAGCCAACTTTACGCCTAGCGTGTAGACTCTTTGCAGCCACATTCCCGCGGTGTAGACACT NGG 10 8 17 8 24 0 100 5 10