Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604130831_nr1l agtttgagagccggctggcggatgcgctgcaggaactgT(/C)gggcccagcatgaggaccaggtggagcagtataagaaggagctggagaagacttattctgc NGG 10 8 17 8 24 0 100 5 10
    240604115238_rxp3 TTCTCTCTCGCTTTTTTTTTTTTTCTTCTTCTTTTTTTTTTTTTTTTTCAAAAGGAGGGGAGAGGGGGTAAAAAAATGCTGCACTGTGCGGCGAGGCCGGTGAGTGAGCGACGCGGAGCCAATCAGCGCCCGCCGTTCCGAAAGTTGCCTTTTATGGCTCGAGTGGCCGC(+ctt)TGTGGCGTCCTATAAAACCCGGCGGCGCAACGCGCAGCCACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCCTTCGTTGCCGGTCCACACCCGCCACCAGGTAAGCAGGGACGCCGGGCCCAGCGGGCCTTCGCTCTCTCGTGGCTAGTACCTCACTGCAGGGTCCTGAGGATCACTCAGAACGGACACCATGGGCGGGTGGAGGGTGGTGCCGGGCCGCGGAGCGGACACTGGCACAGCCAACTTTACGCCTAGCGTGTAGACTCTTTGCAGCCACATTCCCGCGGTGTAGACACT NGG 10 8 17 8 24 0 100 5 10
    240604113333_4idq ACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGTTGAGGCAGCCAGGGCTGGCCTGTACACTGACTTGAGACCAATAAAAGTGCACACCTTAC(+ctt)CTTACACAAACAGCTTGTGGCTCTGTGGCTTTGCTGGGTGTGGGGAGCAGGTTGGGTGGGTGTGGAGCTCTATTGGGGGGGGCATCTAGGGTGGGCTAGGCCTTGCTGATGGTATCTAGTGGGAGGGCTACAGGCCCTTTAATGAAGTCTCACAAATAACCACACCTTCAGACTGTCTTGAAGAGTTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604113317_px7q ACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGTTGAGGCAGCCAG(+ctt)GGCTGGCCTGTACACTGACTTGAGACCAATAAAAGTGCACACCTTACCTTACACAAACAGCTTGTGGCTCTGTGGCTTTGCTGGGTGTGGGGAGCAGGTTGGGTGGGTGTGGAGCTCTATTGGGGGGGGCATCTAGGGTGGGCTAGGCCTTGCTGATGGTATCTAGTGGGAGGGCTACAGGCCCTTTAATGAAGTCTCACAAATAACCACACCTTCAGACTGTCTTGAAGAGTTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604113252_qiaj TTCTCTCTCGCTTTTTTTTTTTTTCTTCTTCTTTTTTTTTTTTTTTTTCAAAAGGAGGGGAGAGGGGGTAAAAAAATGCTGCACTGTGCGGCGAGGCCGGTGAGTGAGCGACGCGGAGCCAATCAGCGCCCGCCGTTCCGAAAGTTGCCTTTTATGGCTCGAGTGGCCGCTGTGGCGTCCTATAAAACCCGGCGGCGCAACGCGCAGCCACTGTCGAGTCGCGTCCACCCGCGA(+ctt)GCACAGCTTCTTTGCAGCTCCTTCGTTGCCGGTCCACACCCGCCACCAGGTAAGCAGGGACGCCGGGCCCAGCGGGCCTTCGCTCTCTCGTGGCTAGTACCTCACTGCAGGGTCCTGAGGATCACTCAGAACGGACACCATGGGCGGGTGGAGGGTGGTGCCGGGCCGCGGAGCGGACACTGGCACAGCCAACTTTACGCCTAGCGTGTAGACTCTTTGCAGCCACATTCCCGCGGTGTAGACACT NGG 10 8 17 8 24 0 100 5 10
    240604113232_e2cr TTCTCTCTCGCTTTTTTTTTTTTTCTTCTTCTTTTTTTTTTTTTTTTTCAAAAGGAGGGGAGAGGGGGTAAAAAAATGCTGCACTGTGCGGCGAGGCCGGTGAGTGAGCGACGCGGAGCCAATCAGCGCCCGCCGTTCCGAAAGTTGCCTTTTATGGCTCGAGTGGCCG(+ctt)CTGTGGCGTCCTATAAAACCCGGCGGCGCAACGCGCAGCCACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCCTTCGTTGCCGGTCCACACCCGCCACCAGGTAAGCAGGGACGCCGGGCCCAGCGGGCCTTCGCTCTCTCGTGGCTAGTACCTCACTGCAGGGTCCTGAGGATCACTCAGAACGGACACCATGGGCGGGTGGAGGGTGGTGCCGGGCCGCGGAGCGGACACTGGCACAGCCAACTTTACGCCTAGCGTGTAGACTCTTTGCAGCCACATTCCCGCGGTGTAGACACT NGG 10 8 17 8 24 0 100 5 10
    240604113020_oxbv ACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGTTGAGGCAGCCAGGGCTGGCCTGTACACTGACTTGAGACCAATA(+ctt)AAAGTGCACACCTTACCTTACACAAACAGCTTGTGGCTCTGTGGCTTTGCTGGGTGTGGGGAGCAGGTTGGGTGGGTGTGGAGCTCTATTGGGGGGGGCATCTAGGGTGGGCTAGGCCTTGCTGATGGTATCTAGTGGGAGGGCTACAGGCCCTTTAATGAAGTCTCACAAATAACCACACCTTCAGACTGTCTTGAAGAGTTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604112927_jgaq ACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGTTGAGGCAGCCAGGGCTGGCCTGTACACTGACTTGAGACCAATA(+ctt)AAAGTGCACACCTTACCTTACACAAACAGCTTGTGGCTCTGTGGCTTTGCTGGGTGTGGGGAGCAGGTTGGGTGGGTGTGGAGCTCTATTGGGGGGGGCATCTAGGGTGGGCTAGGCCTTGCTGATGGTATCTAGTGGGAGGGCTACAGGCCCTTTAATGAAGTCTCACAAATAACCACACCTTCAGACTGTCTTGAAGAGTTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604111136_a4l9 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240604111051_4rdm CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10