Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604071036_zizotyh6 CTCTGAATCTTCTGAGAGTCAGTCGGAATCTGAGAGCGAATCAGCAGGTTT(/G)CAAATCCCAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAAG NGG 10 8 17 8 24 0 100 5 10
    240604071036_zizotwx8 TTTGATCACAGAGAAAAATGGAAACAGGAACCCTCAGAAGATGAACAGGAA(/G)CAAGGCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240604071036_zizos12z CCGAAGAACTGTGAATTAAAGTCTGTAATCATTTTTCCCCCCTCACACACC(/A)GAAGGCAAGTAGCGGGTCTGAGAGTGGGAGCCCAAAAAGAAGAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604071036_zizoqibf CACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACC(/T)TGTCCCCAGAAGGTGCACTGTTTGCTTAAGATCTCTACTTGGGATGAGC NGG 10 8 17 8 24 0 100 5 10
    240604071036_zizopsl1 GGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTCC(/T)GAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604071036_zizoo2oq GAGTCAGTCGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGG(/C)CCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAACGGATAGCT NGG 10 8 17 8 24 0 100 5 10
    240604071036_zizonw9k CAGTCGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTT(/C)CCAGAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAACGGATAGCTGATG NGG 10 8 17 8 24 0 100 5 10
    240604071036_zizonqhd ACAGATTCCCCCTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAAA(/G)AAGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGA NGG 10 8 17 8 24 0 100 5 10
    240604071036_zizonf1o GATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACCATCGCGG(/T)TTTAATATTAAGGAAGAGGTAAGGAAAAAATGTTTTAAGGGCATGCATT NGG 10 8 17 8 24 0 100 5 10
    240604071036_zizon0mt CTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAATAAGGACAAAAA(/T)CCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAGGTATGAGCTA NGG 10 8 17 8 24 0 100 5 10