Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604070528_xiru9dw0 CCAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAACC(/T)GATAGCTGATGTGAAGAAGGTATCTACTTTGCCCTGCAGTACAAATGTG NGG 10 8 17 8 24 0 100 5 10
    240604070528_xiru7r4h ACAGATTCCCCCTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAAA(/G)AAGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGA NGG 10 8 17 8 24 0 100 5 10
    240604070528_xiru7ha7 ATATCCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACC(/T)ATCGCGATTTAATATTAAGGAAGAGGTAAGGAAAAAATGTTTTAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604070528_xiru6rpr TTCAGACTCAGGCAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAA(/G)TGGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240604070528_xiru6h89 TGGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTT(/A)GGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240604070528_xiru5flm GATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACCATCGCGG(/T)TTTAATATTAAGGAAGAGGTAAGGAAAAAATGTTTTAAGGGCATGCATT NGG 10 8 17 8 24 0 100 5 10
    240604070528_xiru4cc2 GAAGGATTCTTTTAGAAAAAAATTACTTTGTTTTGCTTTTGATCACAGAGG(/C)AAAATGGAAACAGGAACCCTCAGAAGATGAACAGGAACAAGGCACCAGT NGG 10 8 17 8 24 0 100 5 10
    240604070528_xiru3dqe CAGATTCCCCCTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAATT(/G)AGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAG NGG 10 8 17 8 24 0 100 5 10
    240604070528_xiru328i CAAAATAAGTCACTCAGCCTCTGAAGAAGCTTCGGGTTCAGACTCAGGCAA(/G)TCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAGTGGACATGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240604070528_xiru327k CTTAATATTTAAGAATTAAAAGATGATGAGAAATAAGGACAAAAGCCAAGG(/A)GGAGGACAGTTCGCTACACAGCAATGCATCGAGGTATGAGCTATCAACT NGG 10 8 17 8 24 0 100 5 10