Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604070517_7jtrhoz1 TTTTGATCACAGAGAAAAATGGAAACAGGAACCCTCAGAAGATGAACAGGG(/A)ACAAGGCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrgflj CACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACC(/T)TGTCCCCAGAAGGTGCACTGTTTGCTTAAGATCTCTACTTGGGATGAGC NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrgcyi ATCACAGAGAAAAATGGAAACAGGAACCCTCAGAAGATGAACAGGAACAAA(/C)GCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrg6mg CCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACCATCC(/T)CGATTTAATATTAAGGAAGAGGTAAGGAAAAAATGTTTTAAGGGCATGC NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrfd0h GGAACAAGGCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGG(/A)CAGAAGACCTGTCCCCAGAAGGTGCACTGTTTGCTTAAGATCTCTACTT NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrfbbp GGAAGTGGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGG(/A)CAGTCGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCC NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtreo86 TCGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCCC(/T)GAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAACGGATAGCTGATGTGA NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrdh9n CTTAATATTTAAGAATTAAAAGATGATGAGAAATAAGGACAAAAGCCAAGG(/A)GGAGGACAGTTCGCTACACAGCAATGCATCGAGGTATGAGCTATCAACT NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrddki AGAAAAAAATTACTTTGTTTTGCTTTTGATCACAGAGAAAAATGGAAACAA(/G)GAACCCTCAGAAGATGAACAGGAACAAGGCACCAGTGCAGAGAGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrchzz CCGAAGAACTGTGAATTAAAGTCTGTAATCATTTTTCCCCCCTCACACACC(/A)GAAGGCAAGTAGCGGGTCTGAGAGTGGGAGCCCAAAAAGAAGAGGCCAG NGG 10 8 17 8 24 0 100 5 10