Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604070517_7jtr7b1w CAGGCGGTCAAACCGAAGCAGACAAGAACCATCGCGATTTAATATTAAGGG(/A)AGAGGTAAGGAAAAAATGTTTTAAGGGCATGCATTTAAACTCCCTATCT NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtr6lfo CAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAGTGGACATGGCAA(/G)CGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTCGGAATCTGAG NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtr3nbk ATATCCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACC(/T)ATCGCGATTTAATATTAAGGAAGAGGTAAGGAAAAAATGTTTTAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtr30wk GTGGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGG(/T)CGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtr0x2n CTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAATAAGGACAAAAA(/T)CCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAGGTATGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtr0c7z ACAAGGCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAA(/G)AAGACCTGTCCCCAGAAGGTGCACTGTTTGCTTAAGATCTCTACTTGGG NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtr09yo CCGAAGAACTGTGAATTAAAGTCTGTAATCATTTTTCCCCCCTCACACACC(/A)GAAGGCAAGTAGCGGGTCTGAGAGTGGGAGCCCAAAAAGAAGAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtr08xe CAGATTCCCCCTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAATT(/G)AGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAG NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe5z5c0 ATATCCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACC(/T)ATCGCGATTTAATATTAAGGAAGAGGTAAGGAAAAAATGTTTTAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604070433_6fe5y5hc ATCACAGAGAAAAATGGAAACAGGAACCCTCAGAAGATGAACAGGAACAAA(/C)GCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAG NGG 10 8 17 8 24 0 100 5 10