Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604070517_7jtrujcy CATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTCGGAA(/C)TCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrsjov AAAATTACTTTGTTTTGCTTTTGATCACAGAGAAAAATGGAAACAGGAACC(/G)CTCAGAAGATGAACAGGAACAAGGCACCAGTGCAGAGAGTGAGCCAGAA NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrsgxs CCCTCCCCCTTAATATTTAAGAATTAAAAGATGATGAGAAATAAGGACAAA(/G)AGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAGGTATGAGC NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrroag TCTTCTGAGAGTCAGTCGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCC(/T)CAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrqcvp TCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCCAGAAGCC(/G)AAAGAGAAGCCAGCCTCTAAGAAGGAACGGATAGCTGATGTGAAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrpyxe CCGAAGAACTGTGAATTAAAGTCTGTAATCATTTTTCCCCCCTCACACACC(/A)GAAGGCAAGTAGCGGGTCTGAGAGTGGGAGCCCAAAAAGAAGAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrp6ru AAACAGGAACCCTCAGAAGATGAACAGGAACAAGGCACCAGTGCAGAGAGG(/C)GAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACCTGTCCCCAGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtroxc8 GAAGGATTCTTTTAGAAAAAAATTACTTTGTTTTGCTTTTGATCACAGAGG(/C)AAAATGGAAACAGGAACCCTCAGAAGATGAACAGGAACAAGGCACCAGT NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrot39 TAAATCTCTCTCTCTCTCAAAATAAGTCACTCAGCCTCTGAAGAAGCTTCC(/T)GGTTCAGACTCAGGCAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAG NGG 10 8 17 8 24 0 100 5 10
    240604070517_7jtrnm83 CTCAGGCAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAGTGGACC(/T)TGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTCGGAA NGG 10 8 17 8 24 0 100 5 10