Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140756_l2wm CTTGTACACAGCAGTCTCTGAAAACTCATTGG(+AGGACA)GTACCCAGTATCGTTTAATTATTAGACCATC NGG 10 8 17 8 24 0 100 5 10
    240613140754_108q TTGGCACAAACTGGAGGATTTTACTGTCCATG(+AGGACA)GTACCTTTGCTGGATCCTTTCCCAAAGACAG NGG 10 8 17 8 24 0 100 5 10
    240613140753_ox53 TAACACTGTCTTTGGGAAAGGATCCAGCAAAG(+AGGACA)GTACCATGGACAGTAAAATCCTCCAGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613140752_1f0l TATGTAGAATATACTGGTCTCCAACTCACAAG(+AGGACA)GATCCACCTGCCTCTGCCTCCTGAGGGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613140751_e7gj CAGTCTCACCCCTCAGGAGGCAGAGGCAGGTG(+AGGACA)GATCCTTGTGAGTTGGAGACCAGTATATTCT NGG 10 8 17 8 24 0 100 5 10
    240613140749_ya0u TGAGCTGAGCGTCTCTATCCTTCCCTGTTCAG(+AGGACA)GATCCTTCCTGCAGGTGCTCATGAGATAGTA NGG 10 8 17 8 24 0 100 5 10
    240613140748_5ufj GATGCTACTATCTCATGAGCACCTGCAGGAAG(+AGGACA)GATCCTGAACAGGGAAGGATAGAGACGCTCA NGG 10 8 17 8 24 0 100 5 10
    240613140747_piy4 CCAGTCTACACAGACAGGCCCCATGGATTCTG(+AGGACA)GTACCTTCAGTTATGGGAGTTATTTGTAATT NGG 10 8 17 8 24 0 100 5 10
    240613140746_twbf ACACAAATTACAAATAACTCCCATAACTGAAG(+AGGACA)GTACCAGAATCCATGGGGCCTGTCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240613140744_j34t CTAGGCAGCTGTATAGAGAAAACTCAGAGGTG(+AGGACA)GTACCTGGTTTCCCATGTGGGTCTAAGCAGT NGG 10 8 17 8 24 0 100 5 10