Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140715_qai2 ACCCTAGCCATTTGGAAAAGACAGGAACTTGG(+AGGACA)GATCCTTGGTGGGTGCTAGTTATTTATGGTA NGG 10 8 17 8 24 0 100 5 10
    240613140714_ouyn GTCAGGAGAACACTTGAAAGGCTTTCTTTGTG(+AGGACA)GTACCTACGGGGTGAAATCACAGCCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240613140712_3j00 CTCTCTTGACAGGCTGTGATTTCACCCCGTAG(+AGGACA)GTACCACAAAGAAAGCCTTTCAAGTGTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613140711_5rsq ATTTGAAATGGTTGATAAAATCCTCAAGCTAG(+AGGACA)GATCCCCCAGAAGGTGTTTGATTGCTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613140709_i99k AAGGGAACAAAGCAATCAAACACCTTCTGGGG(+AGGACA)GATCCTAGCTTGAGGATTTTATCAACCATTT NGG 10 8 17 8 24 0 100 5 10
    240613140707_pogt ATCATCTTACCATTCTGATCCTTCATGCTCTG(+AGGACA)GTACCCGAGCTCGGAGCGCCCCATCCGTCTA NGG 10 8 17 8 24 0 100 5 10
    240613140706_ulfa ACAGGTAGACGGATGGGGCGCTCCGAGCTCGG(+AGGACA)GTACCAGAGCATGAAGGATCAGAATGGTAAG NGG 10 8 17 8 24 0 100 5 10
    240613140705_hstf AACATAATTAGAAAGTCTCCTTCTGGCTGATG(+AGGACA)GTACCACTGGTCTCTCACCACACTAAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613140703_qy1u TGGCAAAACCTTAGTGTGGTGAGAGACCAGTG(+AGGACA)GTACCATCAGCCAGAAGGAGACTTTCTAATT NGG 10 8 17 8 24 0 100 5 10
    240613140701_s6fi GCTAGTCCTATACTTTTACTTAATCTTCTCTG(+AGGACA)GTACCAGGTAATATTCTCTTTCTTTCTCCTG NGG 10 8 17 8 24 0 100 5 10