Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140612_xml9 GAAAAGAAGGTCCACTGACAGGCCTGAAGTGG(+AGGACA)GATCCATCTCAAGGGGAGGACCCAAGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613140611_tqcb CAAGGTTTGAGGATATTTGAATAAAATTTTTG(+AGGACA)GATCCAGCTGCAACATAAAGAGTGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613140610_c466 ATGTGGGAAGGGCACTCTTTATGTTGCAGCTG(+AGGACA)GATCCAAAAATTTTATTCAAATATCCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240613140608_kgaa TGAAGCAATAGAAACATGCGATTTAGCTTTGG(+AGGACA)GATCCTCTGAATGAGGGATTCCCCCAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613140607_ja1n TTATAGCCTTTGGGGGAATCCCTCATTCAGAG(+AGGACA)GATCCCAAAGCTAAATCGCATGTTTCTATTG NGG 10 8 17 8 24 0 100 5 10
    240613140604_utfv AGCCCCCTCCCCCATCGAATCATCCCCCCAGG(+AGGACA)GTACCCCAGCTAGTCACATACAAAACAGTTT NGG 10 8 17 8 24 0 100 5 10
    240613140602_xlza AGACAAAACTGTTTTGTATGTGACTAGCTGGG(+AGGACA)GTACCCTGGGGGGATGATTCGATGGGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613140600_fqsl ACAAAGACAAGGACCAGAAGCAAATACTGGTG(+AGGACA)GATCCAGATGTTTGAAATCTTTGTATCAAAA NGG 10 8 17 8 24 0 100 5 10
    240613140559_0h60 ACACGTTTTGATACAAAGATTTCAAACATCTG(+AGGACA)GATCCACCAGTATTTGCTTCTGGTCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240613140557_rkda GCTCAACAATACAGAGAATATGTGAACCTGAG(+AGGACA)GTACCCTGACTCAAATTTCACAACCACAATG NGG 10 8 17 8 24 0 100 5 10