Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140538_aufj GCTGGGATAGTGAGAAGCATCTGCAATCCCAG(+AGGACA)GTACCTAAGGGTTGACACAGGAGGGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240613140536_y9t1 GAGCATGCAGCTAAGCAGATATGAGCAGGAGG(+AGGACA)GATCCCATGTAAGAAAGTAAGACACCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613140535_ek8g AAAGTCCTCTGGTGTCTTACTTTCTTACATGG(+AGGACA)GATCCCTCCTGCTCATATCTGCTTAGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240613140533_a6hs GCTGTCTTCAGACATACCAGAAGAGGACATAG(+AGGACA)GATCCTCATTTTATGTGGTTGGTAGGAATTG NGG 10 8 17 8 24 0 100 5 10
    240613140532_jp0c GAGTTCAATTCCTACCAACCACATAAAATGAG(+AGGACA)GATCCTATGTCCTCTTCTGGTATGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613140531_ke2m CCTAGCTGGGCTCGACCACTTAGCTGGTGAAG(+AGGACA)GATCCTTCCGAGCCCCCAACCCTACCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240613140530_jsd1 ATCGCTCTGGGGTAGGGTTGGGGGCTCGGAAG(+AGGACA)GATCCTTCACCAGCTAAGTGGTCGAGCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613140528_3fm6 CTTGCCTGCTAGCTTCCCTCAGACGGCTTGGG(+AGGACA)GTACCCTGAGCCACACAAGTGCCAGGATAGC NGG 10 8 17 8 24 0 100 5 10
    240613140526_x2w9 CGCCTGCTATCCTGGCACTTGTGTGGCTCAGG(+AGGACA)GTACCCCAAGCCGTCTGAGGGAAGCTAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613140525_36h9 TTGGGGAGCTGCGAGGAAAGGGCAGTAAGCAG(+AGGACA)GATCCTGGCACGGCAACCTGTATCTCTAGAA NGG 10 8 17 8 24 0 100 5 10