Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140645_f80w ACTTACATGTATGTTCTTAAGAGAAAAACTAG(+AGGACA)GTACCCATAGTGCACGTTTGATTAGGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240613140644_v7b2 TTATTCAAATTTTGATTATTTTAAAAATAAAG(+AGGACA)GATCCATTCTTACCGAAAGGGACACTACATC NGG 10 8 17 8 24 0 100 5 10
    240613140642_oi8n TTAAAGATGTAGTGTCCCTTTCGGTAAGAATG(+AGGACA)GATCCTTTATTTTTAAAATAATCAAAATTTG NGG 10 8 17 8 24 0 100 5 10
    240613140641_8ysz CTCCACTTCCCATCTGACCGCTGATGTGCTGG(+AGGACA)GATCCCAGAGCATACTCCTGAGCCTGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613140639_y8f6 GTCATAAAGCCAGGCTCAGGAGTATGCTCTGG(+AGGACA)GATCCCAGCACATCAGCGGTCAGATGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613140638_jf6g TATGTTAATATGATGAAATAAAATCAGAACTG(+AGGACA)GTACCTATTTGTACGTAAGTGTGAGACGTGG NGG 10 8 17 8 24 0 100 5 10
    240613140636_ae4l AGAAGCCACGTCTCACACTTACGTACAAATAG(+AGGACA)GTACCAGTTCTGATTTTATTTCATCATATTA NGG 10 8 17 8 24 0 100 5 10
    240613140635_mqoi TTCAGTTTCCAGTGACCCACAAAAGGGGCGTG(+AGGACA)GTACCGGAAAAACAAAAAGATGGTGTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240613140633_byd5 CCCTGCCAGAACACCATCTTTTTGTTTTTCCG(+AGGACA)GTACCACGCCCCTTTTGTGGGTCACTGGAAA NGG 10 8 17 8 24 0 100 5 10
    240613140632_5yc7 GTATTTGGCTTAATCTCCCCATTTGATATAAG(+AGGACA)GTACCAGTCATTTTTAGAGGCCAAAAAAAGG NGG 10 8 17 8 24 0 100 5 10