Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140631_lpec GGGTTCCTTTTTTTGGCCTCTAAAAATGACTG(+AGGACA)GTACCTTATATCAAATGGGGAGATTAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613140626_ech4 ACAGTGAATGGGCCAAACTGTGTTTATGGTTG(+AGGACA)GATCCCGAACAGTGTGAAAAGACAAGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613140625_lah3 AATGACCACTCTTGTCTTTTCACACTGTTCGG(+AGGACA)GATCCAACCATAAACACAGTTTGGCCCATTC NGG 10 8 17 8 24 0 100 5 10
    240613140623_yr8z AGTGCCAGGCCTTGGGTCCTCCCCTTGAGATG(+AGGACA)GATCCCACTTCAGGCCTGTCAGTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240613140622_6ijl GAAAAGAAGGTCCACTGACAGGCCTGAAGTGG(+AGGACA)GATCCATCTCAAGGGGAGGACCCAAGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613140620_wc9i GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+AGGACA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613140618_9255 GGATGGACCATGTAGAGACTGCCATATCCAGG(+AGGACA)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240613140616_40bq CAAGGTTTGAGGGTATTTGAATAAAATTTTTG(+AGGACA)GATCCAGCTGCAACATAAAGAGTGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613140615_pumi ATGTGGGAAGGGCACTCTTTATGTTGCAGCTG(+AGGACA)GATCCAAAAATTTTATTCAAATACCCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240613140614_0o1b AGTGCCAGGCCTTGGGTCCTCCCCTTGAGATG(+AGGACA)GATCCCACTTCAGGCCTGTCAGTGGACCTTC NGG 10 8 17 8 24 0 100 5 10