Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140555_k64a GGTTTCATTGTGGTTGTGAAATTTGAGTCAGG(+AGGACA)GTACCTCAGGTTCACATATTCTCTGTATTGT NGG 10 8 17 8 24 0 100 5 10
    240613140554_j8ul AAGACATATACATATATGTTAATCAAATATTG(+AGGACA)GTACCAGAGTACACACTGTGTTTGCATGATT NGG 10 8 17 8 24 0 100 5 10
    240613140552_vxei GAGAAAATCATGCAAACACAGTGTGTACTCTG(+AGGACA)GTACCAATATTTGATTAACATATATGTATAT NGG 10 8 17 8 24 0 100 5 10
    240613140551_qr39 CCAGGTCAGTTGTAGGAATTCTTGAGTATCAG(+AGGACA)GATCCATCGATGTGTAGACAAAACTGTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613140550_3690 CACTCTAAAACAGTTTTGTCTACACATCGATG(+AGGACA)GATCCTGATACTCAAGAATTCCTACAACTGA NGG 10 8 17 8 24 0 100 5 10
    240613140548_lnid TGAACCTGAGACCCTCCTGTGTCAACCCTTCG(+AGGACA)GTACCTGGGATTACAGATGCTTCTCAGGATC NGG 10 8 17 8 24 0 100 5 10
    240613140546_c3yu GCTGGGATCCTGAGAAGCATCTGTAATCCCAG(+AGGACA)GTACCGAAGGGTTGACACAGGAGGGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240613140545_2ogf CTTCGGTACCTGGGATTACAGATGCTTCTCAG(+AGGACA)GATCCCAGCCATTCTGCTTTCATTATTAAAG NGG 10 8 17 8 24 0 100 5 10
    240613140543_7ejr GTAACCTTTAATAATGAAAGCAGAATGGCTGG(+AGGACA)GATCCTGAGAAGCATCTGTAATCCCAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240613140541_gnbz TGAACCTGAGACCCTCCTGTGTCAACCCTTAG(+AGGACA)GTACCTGGGATTGCAGATGCTTCTCACTATC NGG 10 8 17 8 24 0 100 5 10