Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140729_3sny TGGTGGTTGCATCTTCAAACTACATTTGCACG(+AGGACA)GATCCTACGGCAAGCAGGGAAGGGAGAGACT NGG 10 8 17 8 24 0 100 5 10
    240613140728_3tzp CATTTGCTTTCCTTTGCCCTTAAATAAATATG(+AGGACA)GTACCAGAAAGCCCACATTGTGAGTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240613140727_ba11 TGCATAAGACAACTCACAATGTGGGCTTTCTG(+AGGACA)GTACCATATTTATTTAAGGGCAAAGGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240613140725_o0qn ACCAAATATACAGCATCAATAAAATGTACACG(+AGGACA)GTACCATGAAAAATTCGAGAACTCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613140724_lxhh GAAAAGCAGCTTGAGTTCTCGAATTTTTCATG(+AGGACA)GTACCGTGTACATTTTATTGATGCTGTATAT NGG 10 8 17 8 24 0 100 5 10
    240613140723_lbap ACTGCTGGTGTATCTGTGAAGTGTTTGCAGTG(+AGGACA)GATCCATCTGCCGCTGCCTGCTAACATATGA NGG 10 8 17 8 24 0 100 5 10
    240613140722_71b8 TTAGTTCATATGTTAGCAGGCAGCGGCAGATG(+AGGACA)GATCCACTGCAAACACTTCACAGATACACCA NGG 10 8 17 8 24 0 100 5 10
    240613140720_50j8 GTTCCAGGAGCCTCCTCATGTTTGCCTGCTGG(+AGGACA)GATCCCTGCACAAGGTGTTTCTTTACAATAG NGG 10 8 17 8 24 0 100 5 10
    240613140718_4xty CTGGTCTATTGTAAAGAAACACCTTGTGCAGG(+AGGACA)GATCCCAGCAGGCAAACATGAGGAGGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240613140717_swiy AATACTACCATAAATAACTAGCACCCACCAAG(+AGGACA)GATCCCAAGTTCCTGTCTTTTCCAAATGGCT NGG 10 8 17 8 24 0 100 5 10