Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140742_zlxe CCAAGACTGCTTAGACCCACATGGGAAACCAG(+AGGACA)GTACCACCTCTGAGTTTTCTCTATACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613140741_p5af TTTCCTGTCATCAAGAAGCTATCACATTTTTG(+AGGACA)GTACCTCTATAAAGCTAACCCTTCACAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613140740_0ebk CTTTTCTTTTGTGAAGGGTTAGCTTTATAGAG(+AGGACA)GTACCAAAAATGTGATAGCTTCTTGATGACA NGG 10 8 17 8 24 0 100 5 10
    240613140738_142w TGCTAATCTTGGCATGTGCACATTCCTCAGAG(+AGGACA)GTACCAGGTATTCCCAGCCATGCATCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240613140736_vlyg TTCCCACAGAGATGCATGGCTGGGAATACCTG(+AGGACA)GTACCTCTGAGGAATGTGCACATGCCAAGAT NGG 10 8 17 8 24 0 100 5 10
    240613140735_85z7 GACTTGGAACTCCAGACTCAAAGATCAAACAG(+AGGACA)GTACCTGAAGCCCTGCTTGCCTGTTTAATAA NGG 10 8 17 8 24 0 100 5 10
    240613140734_q6yu GTATTTTATTAAACAGGCAAGCAGGGCTTCAG(+AGGACA)GTACCTGTTTGATCTTTGAGTCTGGAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240613140733_pcqg GGTAGAGTGCCCCCAAGTGGCAGGTAGCAGTG(+AGGACA)GTACCAATCATATGACCCAGATAAGCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240613140732_ctmt TAGAGGGAGGGCTTATCTGGGTCATATGATTG(+AGGACA)GTACCACTGCTACCTGCCACTTGGGGGCACT NGG 10 8 17 8 24 0 100 5 10
    240613140730_a1yu GTGACAGTCTCTCCCTTCCCTGCTTGCCGTAG(+AGGACA)GATCCGTGCAAATGTAGTTTGAAGATGCAAC NGG 10 8 17 8 24 0 100 5 10