Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132355_jokv AGGGTTACAGTGATCCTGGTGAGTGACCAGGG(+AGGACA)GTACCCCATCCTGGCACCCCTCCCCTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613132353_fhqb TGGTTACCTTGCCCTGAAAGATGCAGCCAGGG(+AGGACA)GTACCTGGGTTAACCCAAGGGCCCAAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240613132350_mx1i AGACCAGGGCTTGGGCCCTTGGGTTAACCCAG(+AGGACA)GTACCCCTGGCTGCATCTTTCAGGGCAAGGT NGG 10 8 17 8 24 0 100 5 10
    240613132348_tf97 TGGGTGAGGAGGTACACACAGGGCTCAGGATG(+AGGACA)GATCCCCTATGGGGAGGAGGGCAGACAATTT NGG 10 8 17 8 24 0 100 5 10
    240613132347_i68o AGTGCAAATTGTCTGCCCTCCTCCCCATAGGG(+AGGACA)GATCCATCCTGAGCCCTGTGTGTACCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613132346_v7yh TGGGAATCACACTTTAAAAGGGTCAGAATATG(+AGGACA)GATCCCGAGGGAAAGAATGCTCCGCACTGTT NGG 10 8 17 8 24 0 100 5 10
    240613132343_uy4k TCATGAACAGTGCGGAGCATTCTTTCCCTCGG(+AGGACA)GATCCATATTCTGACCCTTTTAAAGTGTGAT NGG 10 8 17 8 24 0 100 5 10
    240613132342_cqn7 TGCAATGCCTCAGAGACAAGAAGAGTGCATTG(+AGGACA)GATCCTAGGGGGCTGCAGTTACAGTCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240613132339_79i6 ACTCACAACAGACTGTAACTGCAGCCCCCTAG(+AGGACA)GATCCAATGCACTCTTCTTGTCTCTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613132337_xxtv TGCAATATTTGTACACATATACAAGTCCTGAG(+AGGACA)GATCCTAATTTCAGCTCAGAGATCGAATTGG NGG 10 8 17 8 24 0 100 5 10