Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132336_bp5k CTGACCCAATTCGATCTCTGAGCTGAAATTAG(+AGGACA)GATCCTCAGGACTTGTATATGTGTACAAATA NGG 10 8 17 8 24 0 100 5 10
    240613132334_f6cn CAAGGAGTTCTAGCATACACTTTTGTGATTGG(+AGGACA)GTACCATATGGTTATATCTGATTCTTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240613132333_9iaz TCATGCTACCAAGAATCAGATATAACCATATG(+AGGACA)GTACCCAATCACAAAAGTGTATGCTAGAACT NGG 10 8 17 8 24 0 100 5 10
    240613132331_5vwp CTTAAAAGGTGGAGTTGTGGGTAGATGAAAAG(+AGGACA)GTACCGTCCAAAAGCAGACTGTAAATTATGA NGG 10 8 17 8 24 0 100 5 10
    240613132330_48sg TGCTGTCATAATTTACAGTCTGCTTTTGGACG(+AGGACA)GTACCTTTTCATCTACCCACAACTCCACCTT NGG 10 8 17 8 24 0 100 5 10
    240613132329_ttus TGCCAGTGAATTCTCTGAGGAAGCCTGCGATG(+AGGACA)GATCCTTTCAGGTGACTTATTACATAGGGTT NGG 10 8 17 8 24 0 100 5 10
    240613132328_0vn8 GAGAGAACCCTATGTAATAAGTCACCTGAAAG(+AGGACA)GATCCATCGCAGGCTTCCTCAGAGAATTCAC NGG 10 8 17 8 24 0 100 5 10
    240613132327_zjjf GAAAGAGTTGGCAATTCAGACTTTCTTGTTTG(+AGGACA)GATCCTATTATCAGCTCCTTAGGGTTTGTAT NGG 10 8 17 8 24 0 100 5 10
    240613132326_pdgb TCAAGATACAAACCCTAAGGAGCTGATAATAG(+AGGACA)GATCCAAACAAGAAAGTCTGAATTGCCAACT NGG 10 8 17 8 24 0 100 5 10
    240613132324_n9ig CCTGCAGTGTATACAAAAGAATTTACTCCTTG(+AGGACA)GATCCCTTGTGTTTGGCTACAGTTACCTGTT NGG 10 8 17 8 24 0 100 5 10