Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132240_snry GGCAGACTCCCAGAGAGCCTGTGAGCTCCAGG(+AGGACA)GTACCCTGAAGCTCCACGGCTGCCGCACATC NGG 10 8 17 8 24 0 100 5 10
    240613132238_vvlg TACATTAGCAGGGGAGGCCTGCAGTAACAGAG(+AGGACA)GATCCTAAAGAATGAACTGGCCGGACTCACT NGG 10 8 17 8 24 0 100 5 10
    240613132237_omas GGAAGAGTGAGTCCGGCCAGTTCATTCTTTAG(+AGGACA)GATCCTCTGTTACTGCAGGCCTCCCCTGCTA NGG 10 8 17 8 24 0 100 5 10
    240613132236_l8h6 CACTCCTCCATTGCTGGTGGGATTGCAAGCTG(+AGGACA)GTACCATCACTCTGGAAATCAGTCTTGCAGT NGG 10 8 17 8 24 0 100 5 10
    240613132234_z8zv TAGGAACTGCAAGACTGATTTCCAGAGTGATG(+AGGACA)GTACCAGCTTGCAATCCCACCAGCAATGGAG NGG 10 8 17 8 24 0 100 5 10
    240613132233_1klb TAACTTTATTTTAAAAAGAAAACAAAGCTTTG(+AGGACA)GATCCCAAACCACAGATTAATGGCTACACAG NGG 10 8 17 8 24 0 100 5 10
    240613132232_bbfd AATCACTGTGTAGCCATTAATCTGTGGTTTGG(+AGGACA)GATCCAAAGCTTTGTTTTCTTTTTAAAATAA NGG 10 8 17 8 24 0 100 5 10
    240613132230_jd8n TCAACTAGTCTTCCTTGTAATCAGAGAAACCG(+AGGACA)GATCCACCAAGTCTTACTCAGCATCCAGCGA NGG 10 8 17 8 24 0 100 5 10
    240613132229_o2yt ACCAATCGCTGGATGCTGAGTAAGACTTGGTG(+AGGACA)GATCCGGTTTCTCTGATTACAAGGAAGACTA NGG 10 8 17 8 24 0 100 5 10
    240613132227_xymj AGTGTTTAACAGCACTGGCTGCTCTTCCAGAG(+AGGACA)GTACCCAGGCTTAATTCCCAGCACCAACATG NGG 10 8 17 8 24 0 100 5 10