Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132308_ne22 TATGAGGCTGTGAGCAGACGTATAGAAGCTGG(+AGGACA)GTACCAAGCCCAGGTCTTCTGTAGGAACATT NGG 10 8 17 8 24 0 100 5 10
    240613132306_3wox GGCTGGAACATTCACTTTGTACAGTACCATTG(+AGGACA)GTACCAGCAAGAAATATCCACAGGAATAAAT NGG 10 8 17 8 24 0 100 5 10
    240613132305_b3ir ATTTCATTTATTCCTGTGGATATTTCTTGCTG(+AGGACA)GTACCAATGGTACTGTACAAAGTGAATGTTC NGG 10 8 17 8 24 0 100 5 10
    240613132304_vngf TATCTTCAGGAGATATGTTACTGCACTTGCAG(+AGGACA)GTACCTTAATTCACTTGAACATTTACAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613132302_ib5w AGTGTCCTTTGTAAATGTTCAAGTGAATTAAG(+AGGACA)GTACCTGCAAGTGCAGTAACATATCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613132301_7v5t ATAAACAACAAAGCTAGTCATTTTGTCCTATG(+AGGACA)GATCCTGTCCTCACATTAAACACTGTCCAGT NGG 10 8 17 8 24 0 100 5 10
    240613132300_bt0k CCGCCACTGGACAGTGTTTAATGTGAGGACAG(+AGGACA)GATCCATAGGACAAAATGACTAGCTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240613132259_hpuc ACCCTTTTCTTAGGGCACACAAATTAATCTAG(+AGGACA)GTACCTTGTTGTGTACAATTGAGAACACCTT NGG 10 8 17 8 24 0 100 5 10
    240613132257_gu9o AAGTCAAGGTGTTCTCAATTGTACACAACAAG(+AGGACA)GTACCTAGATTAATTTGTGTGCCCTAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613132256_4p5k AAAGCAAATGGCAGAGCCTTTTGATATCAGAG(+AGGACA)GATCCTCTTACACTTCTTCCCCCCTCCATTG NGG 10 8 17 8 24 0 100 5 10