Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132207_7kit GAAGCTGCTAGCCAGGGTCTTCCTGATAAAAG(+AGGACA)GTACCTGGGTTCCCTGCCACCGAGTCCATAG NGG 10 8 17 8 24 0 100 5 10
    240613132206_d16x GAAACTGCTTGTGCATTGAAACAGAATTCAGG(+AGGACA)GTACCTTCTATCTGTGTTTGTGGGCCATTGC NGG 10 8 17 8 24 0 100 5 10
    240613132204_2o5b GAGTGGCAATGGCCCACAAACACAGATAGAAG(+AGGACA)GTACCCTGAATTCTGTTTCAATGCACAAGCA NGG 10 8 17 8 24 0 100 5 10
    240613132203_zxkr ATAGCATCCTGGTGAAATGAGCTATGTCTCAG(+AGGACA)GTACCACTAAACATCAGCCACTCACCTTTAG NGG 10 8 17 8 24 0 100 5 10
    240613132202_mxru CTGGCCTAAAGGTGAGTGGCTGATGTTTAGTG(+AGGACA)GTACCTGAGACATAGCTCATTTCACCAGGAT NGG 10 8 17 8 24 0 100 5 10
    240613132200_bevh CTTTATGGTCAAGAGATTATTATGACACAGGG(+AGGACA)GTACCATTAGCGTAACAGCCCTCCAGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240613132159_59z3 TGAGCACTCTCTGGAGGGCTGTTACGCTAATG(+AGGACA)GTACCCCTGTGTCATAATAATCTCTTGACCA NGG 10 8 17 8 24 0 100 5 10
    240613132157_iufx GGCACATGGGTCAGAACAAAGTTGCATTATGG(+AGGACA)GTACCAGCGATCCAATTAATTAATATTTATA NGG 10 8 17 8 24 0 100 5 10
    240613132156_oyoi CCAGCTATAAATATTAATTAATTGGATCGCTG(+AGGACA)GTACCCATAATGCAACTTTGTTCTGACCCAT NGG 10 8 17 8 24 0 100 5 10
    240613132155_6y7m GTCATGGGCACTGTGGCTGAAGTGGCTGCTTG(+AGGACA)GTACCAGCCGTCTCAGTAGCAGGAGTGAGCC NGG 10 8 17 8 24 0 100 5 10