Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132114_ub4j GAGGTTGTCTCTGGCTCTGTTGTCTGCTGTTG(+AGGACA)GATCCCTTTCTCCTACCTGGACTGTCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613132112_epm2 GGCCCAACCAGACAGTCCAGGTAGGAGAAAGG(+AGGACA)GATCCAACAGCAGACAACAGAGCCAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240613132111_3lih GGAAGAAGAACCTTTGTAACCTGTTAATGGAG(+AGGACA)GATCCTTATAAAACAGAACAACTCTGTATAA NGG 10 8 17 8 24 0 100 5 10
    240613132110_l6s3 CCGGTTTATACAGAGTTGTTCTGTTTTATAAG(+AGGACA)GATCCTCCATTAACAGGTTACAAAGGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240613132108_693t TGGGACACGCCCCCCCCCCCCCACTTGAGCTG(+AGGACA)GATCCCACTTTGTGCCTTTCTCTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240613132107_ft3d GAAAAGAAGGTCCAGAGAAAGGCACAAAGTGG(+AGGACA)GATCCAGCTCAAGTGGGGGGGGGGGGGCGTG NGG 10 8 17 8 24 0 100 5 10
    240613132106_yfzm ATGGTAATAAATAAATAAGATTTCTTTTAATG(+AGGACA)GATCCAGACTTACCTGAGGACAACTGTAGGG NGG 10 8 17 8 24 0 100 5 10
    240613132104_xlu0 ATTAACCCTACAGTTGTCCTCAGGTAAGTCTG(+AGGACA)GATCCATTAAAAGAAATCTTATTTATTTATT NGG 10 8 17 8 24 0 100 5 10
    240613132102_oiwz GATCAGCATCCAGGCCCATCCATCAGCCTTCG(+AGGACA)GATCCTGCTCTGCTCTTTATTCTGCTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240613132101_yu2x GGAGTGAGACAGCAGAATAAAGAGCAGAGCAG(+AGGACA)GATCCGAAGGCTGATGGATGGGCCTGGATGC NGG 10 8 17 8 24 0 100 5 10