Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132009_ps6w ACCGTGTGATAAGGTTGTGTGCTGTGTGATCG(+AGGACA)GTACCCAGGGAGCGGGGCGGTGTTAATGTGC NGG 10 8 17 8 24 0 100 5 10
    240613132006_6dt2 ATTTGGCACATTAACACCGCCCCGCTCCCTGG(+AGGACA)GTACCGATCACACAGCACACAACCTTATCAC NGG 10 8 17 8 24 0 100 5 10
    240613132005_5nvl TTGCTGATGCTCAGTGGTCCAGGCTACGCTGG(+AGGACA)GATCCGTGATGGGGATATCCCAGAAGACACC NGG 10 8 17 8 24 0 100 5 10
    240613132004_fzb3 GGAATGGTGTCTTCTGGGATATCCCCATCACG(+AGGACA)GATCCCAGCGTAGCCTGGACCACTGAGCATC NGG 10 8 17 8 24 0 100 5 10
    240613132003_8klx ACAGCAATCCATGGCTTTCCAGAAGAGGTGTG(+AGGACA)GATCCAAAAGTGCCACCTCTATCTTAGTCAC NGG 10 8 17 8 24 0 100 5 10
    240613132002_h9fb TGCTGGTGACTAAGATAGAGGTGGCACTTTTG(+AGGACA)GATCCACACCTCTTCTGGAAAGCCATGGATT NGG 10 8 17 8 24 0 100 5 10
    240613132000_vazn TCAAGTGCCAAAGCTGTCTCGTGAGCTTCCAG(+AGGACA)GATCCTGACAGGCCTGGAGGGAACTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240613131958_u2ej TGGCCCTTTGCAGTTCCCTCCAGGCCTGTCAG(+AGGACA)GATCCTGGAAGCTCACGAGACAGCTTTGGCA NGG 10 8 17 8 24 0 100 5 10
    240613131957_xg2e CAGGTGTTCTTTCAGGCTGGTCAGGAGCTGTG(+AGGACA)GTACCAGGGACAAGGCCTGCCTCTTGTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613131954_n2bu AAAACAAAGACAAGAGGCAGGCCTTGTCCCTG(+AGGACA)GTACCACAGCTCCTGACCAGCCTGAAAGAAC NGG 10 8 17 8 24 0 100 5 10