Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613131907_oxop TTGTAAGAAGCATTTTAAGCAGCTGCTGTAGG(+AGGACA)GTACCTTAAAAAAAAAACTTCCAGTAGAGTA NGG 10 8 17 8 24 0 100 5 10
    240613131906_6i3g ATTTATACTCTACTGGAAGTTTTTTTTTTAAG(+AGGACA)GTACCCTACAGCAGCTGCTTAAAATGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613131903_xluh CTCGAGAAACGCAGTCTCGGCCTCCTCCCAGG(+AGGACA)GATCCAAACGCCAGCCACCCGCCCGGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240613131902_k4bn TCTCTAAAAGCCGGGCGGGTGGCTGGCGTTTG(+AGGACA)GATCCCTGGGAGGAGGCCGAGACTGCGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613131900_cm07 GACTGTGAATGAAGAGCTGTGCTGTGTGGGTG(+AGGACA)GTACCTGCTGTCTCCTATCTGCTGGGTCTTA NGG 10 8 17 8 24 0 100 5 10
    240613131859_4avl AGATCTAAGACCCAGCAGATAGGAGACAGCAG(+AGGACA)GTACCACCCACACAGCACAGCTCTTCATTCA NGG 10 8 17 8 24 0 100 5 10
    240613131857_qe9g GATGTTGCTGCTGTTAGGAAGGTAGGCAGAAG(+AGGACA)GATCCATGTCTGTGCCGAGAGGTGTGACGCC NGG 10 8 17 8 24 0 100 5 10
    240613131856_46a8 CCCCAGGCGTCACACCTCTCGGCACAGACATG(+AGGACA)GATCCTTCTGCCTACCTTCCTAACAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613131855_8jq3 CTCCTGTCGGGTATTTCTTACTGGCACAGTAG(+AGGACA)GTACCTGGATGTTCACAGAGCATCCGCCCCG NGG 10 8 17 8 24 0 100 5 10
    240613131853_hgdz TGGGACGGGGCGGATGCTCTGTGAACATCCAG(+AGGACA)GTACCTACTGTGCCAGTAAGAAATACCCGAC NGG 10 8 17 8 24 0 100 5 10